{"id":"1f69db4e-8188-5abd-8983-8b90404cf917","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-bilirubin-unbound-rat","predicate":"increases_in_adult_rats","statement":"Repeated intraperitoneal berberine increased unbound bilirubin concentrations in adult rats.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"73d92a24-fb47-50b3-b1aa-188bc96137d6","mechanism_event_label":"The laboratory binding concern also appeared in an animal exposure model.","subject":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"object":{"id":"7d7ca216-0687-5844-80d8-8b2899887b3b","slug":"unbound-bilirubin-concentration","display_name":"Unbound bilirubin concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"73d92a24-fb47-50b3-b1aa-188bc96137d6","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-bilirubin-unbound-rat-event","event_type":"biochemical_relationship","label":"The laboratory binding concern also appeared in an animal exposure model.","description":"Repeated intraperitoneal berberine increased unbound bilirubin concentrations in adult rats.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7ad4dc4f-afe1-5810-8a07-2e23c421f1ce","slug":"bilirubin","display_name":"Bilirubin","entity_type_key":"small_molecule"},"role":"measured_ligand","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7d7ca216-0687-5844-80d8-8b2899887b3b","slug":"unbound-bilirubin-concentration","display_name":"Unbound bilirubin concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/berberine-research/8513024.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6ff3832e3aad7806081c5b3bd378eabf1595da6dd2a1f3bef664bb69c9d11334\", \"start_char\": 0, \"end_char\": 1066, \"text_sha256\": \"6ff3832e3aad7806081c5b3bd378eabf1595da6dd2a1f3bef664bb69c9d11334\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bilirubin-binding assay and adult-rat exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Binding displacement assay; intraperitoneal berberine for one week in rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No infant risk incidence was measured. Adult-rat parenteral exposure is not equivalent to oral adult supplementation; protein displacement is a biologically relevant concern for neonatal jaundice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Berberine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"In-vitro protein binding and adult rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The laboratory binding concern also appeared in an animal exposure model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[berberine-p8513024] Displacement of bilirubin from albumin by berberine. (1993). https://pubmed.ncbi.nlm.nih.gov/8513024/ DOI: 10.1159/000243932","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Albumin-bound versus unbound bilirubin","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9b761ace-d6c0-5fd0-a8a7-a64f0887d994","evidence_kind":"source_excerpt","locator":"Lines 974-985","start_line":974,"end_line":985,"excerpt":"### berberine-bilirubin-unbound-rat\nRepeated intraperitoneal berberine increased unbound bilirubin concentrations in adult rats.\nCondition category: normal\nnutrient_topic: Berberine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The laboratory binding concern also appeared in an animal exposure model.\norganism: In-vitro protein binding and adult rats\ntissue_or_cell_type: Albumin-bound versus unbound bilirubin\nexperimental_model: Bilirubin-binding assay and adult-rat exposure\nlimitations: No infant risk incidence was measured. Adult-rat parenteral exposure is not equivalent to oral adult supplementation; protein displacement is a biologically relevant concern for neonatal jaundice.\nexposure: Binding displacement assay; intraperitoneal berberine for one week in rats\nevidence_span: {\"source_cache\": \"artifacts/berberine-research/8513024.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6ff3832e3aad7806081c5b3bd378eabf1595da6dd2a1f3bef664bb69c9d11334\", \"start_char\": 0, \"end_char\": 1066, \"text_sha256\": \"6ff3832e3aad7806081c5b3bd378eabf1595da6dd2a1f3bef664bb69c9d11334\"}\n[berberine-p8513024] Displacement of bilirubin from albumin by berberine. (1993). https://pubmed.ncbi.nlm.nih.gov/8513024/ DOI: 10.1159/000243932","model_system":"Bilirubin-binding assay and adult-rat exposure","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [berberine-p8513024] Displacement of bilirubin from albumin by berberine. (1993). https://pubmed.ncbi.nlm.nih.gov/8513024/ DOI: 10.1159/000243932","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9bb61e1d-e5cf-5b05-a9ca-409291f7a313","stable_key":"import-7484635e-cc8f-5cd6-a978-6dc5775a7db5","title":"Berberine: metabolism, nutrient connections and drug interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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