{"id":"1f4f7ee4-334c-5f26-bc93-6d871eb02cce","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-asct1-substrate","predicate":"transports","statement":"Cloned human ASCT1 transported alanine, serine, cysteine and threonine rather than glutamate or aspartate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8e059f70-3f9e-52dd-a529-81078410ec31","mechanism_event_label":"Several amino acids share a cellular entry and exchange route.","subject":{"id":"5a802528-9866-5200-89fe-d32e234882e1","slug":"slc1a4","display_name":"Human neutral amino acid exchanger ASCT1 / SLC1A4","entity_type_key":"protein"},"object":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8e059f70-3f9e-52dd-a529-81078410ec31","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-asct1-substrate-event","event_type":"observed_relationship","label":"Several amino acids share a cellular entry and exchange route.","description":"Cloned human ASCT1 transported alanine, serine, cysteine and threonine rather than glutamate or aspartate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5a802528-9866-5200-89fe-d32e234882e1","slug":"slc1a4","display_name":"Human neutral amino acid exchanger ASCT1 / SLC1A4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cDNA cloning and transporter characterization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Substrate overlap alone does not quantify competition during ordinary dietary intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Several amino acids share a cellular entry and exchange route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Human neutral amino acid transporter ASCT1: structure of the gene (SLC1A4) and localization to chromosome 2p13-p15. · 1994 · https://pubmed.ncbi.nlm.nih.gov/7896285/ · DOI 10.1006/geno.1994.1577","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"depends","comparator":null,"unit":null,"notes":"A substrate-selectivity record. ASCT1 is an obligatory exchanger and no direction is stated.","entity":null},{"dimension":"transport_pool","value_text":"the cytosol across the plasma membrane","comparator":null,"unit":null,"notes":"A substrate-selectivity record. ASCT1 is an obligatory exchanger and no direction is stated.","entity":null}],"evidence":[{"id":"f03a91dc-8b39-5264-b05f-b88fce19a35c","evidence_kind":"source_excerpt","locator":"Lines 18-24","start_line":18,"end_line":24,"excerpt":"## l-threonine-asct1-substrate\nSeveral amino acids share a cellular entry and exchange route.\nCloned human ASCT1 transported alanine, serine, cysteine and threonine rather than glutamate or aspartate.\nModel: Human cDNA cloning and transporter characterization.\nLimitations: Substrate overlap alone does not quantify competition during ordinary dietary intake.\nEvidence access: Primary abstract\nHuman neutral amino acid transporter ASCT1: structure of the gene (SLC1A4) and localization to chromosome 2p13-p15. · 1994 · https://pubmed.ncbi.nlm.nih.gov/7896285/ · DOI 10.1006/geno.1994.1577","model_system":"Human cDNA cloning and transporter characterization.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3365aed939d9f567bd098449c7d67c7c00fb6c9ba163dcf34dc7cf6081f324fb","revision_id":"10a7b648-8dd7-5d4e-b618-c560a6e2d3c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}