{"id":"1f287243-1905-5568-9296-c2d779d23465","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-csad-cysteinesulfinate","predicate":"decarboxylates_to","statement":"Purified human CSAD decarboxylated L-cysteine sulfinic acid to hypotaurine in PLP-containing enzyme assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"588ee4f3-2295-5da1-9213-832f77df856b","mechanism_event_label":"Vitamin B6-dependent CSAD performs a step between cysteine and taurine.","subject":{"id":"a5ef2431-b1fa-57c9-a6ea-c877d4a1e3ec","slug":"csad","display_name":"Human cysteine sulfinic acid decarboxylase / CSAD","entity_type_key":"protein"},"object":{"id":"eeff5174-9aa8-5f34-b107-e826edde648c","slug":"hypotaurine","display_name":"Hypotaurine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"588ee4f3-2295-5da1-9213-832f77df856b","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-csad-cysteinesulfinate-event","event_type":"observed_relationship","label":"Vitamin B6-dependent CSAD performs a step between cysteine and taurine.","description":"Purified human CSAD decarboxylated L-cysteine sulfinic acid to hypotaurine in PLP-containing enzyme assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a5ef2431-b1fa-57c9-a6ea-c877d4a1e3ec","slug":"csad","display_name":"Human cysteine sulfinic acid decarboxylase / CSAD","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eeff5174-9aa8-5f34-b107-e826edde648c","slug":"hypotaurine","display_name":"Hypotaurine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"275dfe52-eab2-5e9d-9618-44c22a6a26d3","slug":"cysteine-sulfinic-acid","display_name":"L-Cysteine sulfinic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, introduction and enzyme-assay results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human enzyme; substrate kinetics and circular-dichroism assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Hypotaurine still needs oxidation to become taurine; this is not a human supplementation experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Vitamin B6-dependent CSAD performs a step between cysteine and taurine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Novel, Easy Assay Method for Human Cysteine Sulfinic Acid Decarboxylase. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34068845/ · DOI 10.3390/life11050438","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a6e0d6d5-c355-539f-95f7-3dac594ce002","evidence_kind":"source_excerpt","locator":"Lines 33-39","start_line":33,"end_line":39,"excerpt":"## taurine-csad-cysteinesulfinate\nVitamin B6-dependent CSAD performs a step between cysteine and taurine.\nPurified human CSAD decarboxylated L-cysteine sulfinic acid to hypotaurine in PLP-containing enzyme assays.\nModel: Recombinant human enzyme; substrate kinetics and circular-dichroism assays.\nLimitations: Hypotaurine still needs oxidation to become taurine; this is not a human supplementation experiment.\nEvidence access: Primary full text, introduction and enzyme-assay results\nA Novel, Easy Assay Method for Human Cysteine Sulfinic Acid Decarboxylase. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34068845/ · DOI 10.3390/life11050438","model_system":"Recombinant human enzyme; substrate kinetics and circular-dichroism assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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