{"id":"1ef9244a-90da-5618-b2e8-af8dc7cf0ced","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-fpgs-mitochondrial-trapping","predicate":"supports","statement":"Mitochondrial human FPGS maintained a separately retained mitochondrial folate-polyglutamate pool in reconstituted AUXB1 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d7c86de1-c6dc-5dcc-8ad5-d56728d56139","mechanism_event_label":"Mitochondria make and keep their own folate tails.","subject":{"id":"82865b74-bdad-520f-949b-7a60df5c08d5","slug":"fpgs-mitochondrial","display_name":"Human mitochondrial FPGS isoform","entity_type_key":"protein"},"object":{"id":"9f264b15-cef4-5361-9236-edf56e03c511","slug":"mitochondrial-folate-accumulation","display_name":"Mitochondrial folate accumulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d7c86de1-c6dc-5dcc-8ad5-d56728d56139","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-fpgs-mitochondrial-trapping-event","event_type":"biochemical_relationship","label":"Mitochondria make and keep their own folate tails.","description":"Mitochondrial human FPGS maintained a separately retained mitochondrial folate-polyglutamate pool in reconstituted AUXB1 cells.","status":"provisional","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"82865b74-bdad-520f-949b-7a60df5c08d5","slug":"fpgs-mitochondrial","display_name":"Human mitochondrial FPGS isoform","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9f264b15-cef4-5361-9236-edf56e03c511","slug":"mitochondrial-folate-accumulation","display_name":"Mitochondrial folate accumulation","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"81c45abb-713d-521f-9854-23288f880831","slug":"folate-polyglutamates","display_name":"Folate polyglutamates","entity_type_key":"chemical_species"},"role":"retained pool","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human FPGS isoforms in Chinese hamster AUXB1 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Induced mitochondrial FPGS and fractionation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Compartment separation is specific to the validated fractionation experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human enzyme in Cricetulus griseus cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mitochondria make and keep their own folate tails.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"AUXB1 mitochondria","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1269a242-87ef-50b3-993b-051a05d65353","evidence_kind":"source_excerpt","locator":"Lines 291-301","start_line":291,"end_line":301,"excerpt":"### folate-fpgs-mitochondrial-trapping\nMitochondrial human FPGS maintained a separately retained mitochondrial folate-polyglutamate pool in reconstituted AUXB1 cells.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Mitochondria make and keep their own folate tails.\norganism: Human enzyme in Cricetulus griseus cells\ntissue_or_cell_type: AUXB1 mitochondria\nexperimental_model: Human FPGS isoforms in Chinese hamster AUXB1 cells\nlimitations: Compartment separation is specific to the validated fractionation experiment.\nexposure: Induced mitochondrial FPGS and fractionation\n[lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","model_system":"Human FPGS isoforms in Chinese hamster AUXB1 cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lawrence2014] Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase maintain the subcellular compartmentalization of folates (2014). https://pubmed.ncbi.nlm.nih.gov/25164808/ DOI: 10.1074/jbc.m114.593244","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}