{"id":"1e7a9ec5-c8f8-55f3-b1b5-6a035d82bda1","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-baat-taurine","predicate":"conjugates_to","statement":"Expression of cloned human BAAT produced bile acid conjugation activity using taurine, establishing that the enzyme can make taurine-conjugated cholic acid from its activated CoA substrate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6932b744-b867-581d-b302-5f3c7115942e","mechanism_event_label":"Taurine becomes chemically attached to bile acids.","subject":{"id":"b1127d16-82be-5dd0-90db-aa1af23011f0","slug":"baat","display_name":"Human bile acid-CoA:amino acid N-acyltransferase / BAAT","entity_type_key":"protein"},"object":{"id":"4f4ae3d8-b415-53b7-8262-46d90815a0c9","slug":"taurocholate","display_name":"Taurocholate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6932b744-b867-581d-b302-5f3c7115942e","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-baat-taurine-event","event_type":"observed_relationship","label":"Taurine becomes chemically attached to bile acids.","description":"Expression of cloned human BAAT produced bile acid conjugation activity using taurine, establishing that the enzyme can make taurine-conjugated cholic acid from its activated CoA substrate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b1127d16-82be-5dd0-90db-aa1af23011f0","slug":"baat","display_name":"Human bile acid-CoA:amino acid N-acyltransferase / BAAT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4f4ae3d8-b415-53b7-8262-46d90815a0c9","slug":"taurocholate","display_name":"Taurocholate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8d02cbb8-2d1f-5dc6-95ad-20c1e3d33724","slug":"choloyl-coa","display_name":"Choloyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human liver cDNA expressed in bacteria; activity compared with purified liver enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Conjugated bile acid and free taurine are separate molecules.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Taurine becomes chemically attached to bile acids.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glycine and taurine conjugation of bile acids by a single enzyme. Molecular cloning and expression of human liver bile acid CoA:amino acid N-acyltransferase. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8034703/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"be25a183-ba12-52f0-adc7-e7190bb9a78d","evidence_kind":"source_excerpt","locator":"Lines 297-303","start_line":297,"end_line":303,"excerpt":"## taurine-baat-taurine\nTaurine becomes chemically attached to bile acids.\nExpression of cloned human BAAT produced bile acid conjugation activity using taurine, establishing that the enzyme can make taurine-conjugated cholic acid from its activated CoA substrate.\nModel: Human liver cDNA expressed in bacteria; activity compared with purified liver enzyme.\nLimitations: Conjugated bile acid and free taurine are separate molecules.\nEvidence access: Primary abstract\nGlycine and taurine conjugation of bile acids by a single enzyme. Molecular cloning and expression of human liver bile acid CoA:amino acid N-acyltransferase. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8034703/","model_system":"Human liver cDNA expressed in bacteria; activity compared with purified liver enzyme.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c6cb77619a374d9b1b04830032fdd3b33d269d160df5baba3778fad5adf57db5","revision_id":"cc45c491-cc6a-5426-ab72-d7c6d26c3614","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}