{"id":"1e643549-c291-5296-84cd-4f41a10dc77a","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-macrophage-loss-nlrp3","predicate":"restrains","statement":"K-free medium triggered NLRP3-dependent IL-1beta release in primed macrophages, accompanying intracellular K loss.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8cb1c138-f173-5afb-8ce4-fb5723a6a7bb","mechanism_event_label":"Experimentally losing cellular potassium could trigger this immune sensor.","subject":{"id":"8c7b3fef-22fb-5b65-9b51-59f776234bc3","slug":"intracellular-potassium-concentration","display_name":"Intracellular potassium concentration","entity_type_key":"cellular_process"},"object":{"id":"a732d76c-af99-5440-b172-400274462345","slug":"nlrp3-inflammasome","display_name":"NLRP3 inflammasome","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"8cb1c138-f173-5afb-8ce4-fb5723a6a7bb","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-macrophage-loss-nlrp3-event","event_type":"biochemical_relationship","label":"Experimentally losing cellular potassium could trigger this immune sensor.","description":"K-free medium triggered NLRP3-dependent IL-1beta release in primed macrophages, accompanying intracellular K loss.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b53e832d-0776-5495-b5cd-cb6a8e8c538b","slug":"nlrp3","display_name":"NLRP3","entity_type_key":"protein"},"role":"sensor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e9fa1ab4-707e-5ff9-9d60-11f503b54c58","slug":"il1b","display_name":"Interleukin-1 beta / IL1B","entity_type_key":"cytokine"},"role":"readout","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b8161b1e-3f89-55ee-b2c2-5aa83ed0d9ff","slug":"casp1","display_name":"Caspase-1 / CASP1","entity_type_key":"protein"},"role":"processing machinery","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8c7b3fef-22fb-5b65-9b51-59f776234bc3","slug":"intracellular-potassium-concentration","display_name":"Intracellular potassium concentration","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"a732d76c-af99-5440-b172-400274462345","slug":"nlrp3-inflammasome","display_name":"NLRP3 inflammasome","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; 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Study references: [munoz-2013-nlrp3] K+ efflux is the Common Trigger of NLRP3 inflammasome Activation by Bacterial Toxins and Particulate Matter (2013). https://pmc.ncbi.nlm.nih.gov/articles/PMC3730833/ DOI: 10.1016/j.immuni.2013.05.016","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}