{"id":"1dff82ed-f83e-5409-a5e2-c5ad43bc153e","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-stra8-premeiotic-replication","predicate":"deletion_prevents","statement":"Stra8-null female embryonic germ cells failed premeiotic DNA replication despite normal earlier mitotic development.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c7a8030f-5c34-5f7d-bcad-c7379689306d","mechanism_event_label":"STRA8 acts before the ovarian cell copies its DNA for meiosis.","subject":{"id":"463b946a-3c0a-55c1-b44b-1d16b10a3d60","slug":"mouse-stra8-gene","display_name":"Mouse Stra8 gene","entity_type_key":"gene"},"object":{"id":"81a40d57-3b19-5b32-acc1-2be9536ab028","slug":"mouse-ovarian-premeiotic-dna-replication","display_name":"Mouse ovarian premeiotic DNA replication","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c7a8030f-5c34-5f7d-bcad-c7379689306d","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-stra8-premeiotic-replication-event","event_type":"biochemical_relationship","label":"STRA8 acts before the ovarian cell copies its DNA for meiosis.","description":"Stra8-null female embryonic germ cells failed premeiotic DNA replication despite normal earlier mitotic development.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cdfcd658-889e-5002-8167-15af0f8130ae","slug":"mouse-stra8","display_name":"Mouse STRA8","entity_type_key":"protein"},"role":"missing_regulator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"463b946a-3c0a-55c1-b44b-1d16b10a3d60","slug":"mouse-stra8-gene","display_name":"Mouse Stra8 gene","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"81a40d57-3b19-5b32-acc1-2be9536ab028","slug":"mouse-ovarian-premeiotic-dna-replication","display_name":"Mouse ovarian premeiotic DNA replication","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Targeted Stra8 disruption","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Stra8-null versus control embryos; no nutrient withdrawal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Female replication result; male knockout phenotypes cannot be inferred from it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"STRA8 acts before the ovarian cell copies its DNA for meiosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[baltus2006] In germ cells of mouse embryonic ovaries, the decision to enter meiosis precedes premeiotic DNA replication. (2006). https://pubmed.ncbi.nlm.nih.gov/17115059/ DOI: 10.1038/ng1919","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"fetal ovary","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"b5e01024-937d-5592-9d56-e346fb75949f","evidence_kind":"source_excerpt","locator":"Lines 213-224","start_line":213,"end_line":224,"excerpt":"### va-repro-stra8-premeiotic-replication\nStra8-null female embryonic germ cells failed premeiotic DNA replication despite normal earlier mitotic development.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: STRA8 acts before the ovarian cell copies its DNA for meiosis.\norganism: Mus musculus\ntissue_or_cell_type: fetal ovary\nexperimental_model: Targeted Stra8 disruption\nlimitations: Female replication result; male knockout phenotypes cannot be inferred from it.\nexposure: Stra8-null versus control embryos; no nutrient withdrawal.\ncross_nutrient: false\n[baltus2006] In germ cells of mouse embryonic ovaries, the decision to enter meiosis precedes premeiotic DNA replication. (2006). https://pubmed.ncbi.nlm.nih.gov/17115059/ DOI: 10.1038/ng1919","model_system":"Targeted Stra8 disruption","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [baltus2006] In germ cells of mouse embryonic ovaries, the decision to enter meiosis precedes premeiotic DNA replication. (2006). https://pubmed.ncbi.nlm.nih.gov/17115059/ DOI: 10.1038/ng1919","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"83131a07-353f-5301-9ce6-50754c194952","stable_key":"import-3c852f36-7951-5fd9-9013-d771a8d16d8a","title":"Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"559cb4dc3f59519ecb0bc455dbcc73346fbf8e0c51e133f455781f51e4ed07ab","revision_id":"225fe4da-2d21-5c19-a92c-80a5f3cac8cb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}