{"id":"1db28829-7fc4-5f8e-98f2-082f54bba513","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-gdh-oxidation","predicate":"oxidatively_deaminates_to","statement":"The GLUD1 study identifies glutamate oxidation to 2-oxoglutarate as the enzyme reaction underlying its metabolic and regulatory investigation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2f707567-8bf4-5767-846b-0926daa997da","mechanism_event_label":"Removing amino nitrogen connects glutamate with the central carbon cycle.","subject":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"object":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"2f707567-8bf4-5767-846b-0926daa997da","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-gdh-oxidation-event","event_type":"observed_relationship","label":"Removing amino nitrogen connects glutamate with the central carbon cycle.","description":"The GLUD1 study identifies glutamate oxidation to 2-oxoglutarate as the enzyme reaction underlying its metabolic and regulatory investigation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Established reaction described in a primary human GLUD1 genetic/functional study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reaction background is distinguished from the directly measured mutant regulation; this record does not quantify tissue flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Removing amino nitrogen connects glutamate with the central carbon cycle.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Hyperinsulinism and hyperammonemia in infants with regulatory mutations of the glutamate dehydrogenase gene. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9571255/ · DOI 10.1056/NEJM199805073381904","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b0e41221-a1d5-5ba8-9ce5-f9454993729a","evidence_kind":"source_excerpt","locator":"Lines 162-168","start_line":162,"end_line":168,"excerpt":"## glutamate-gdh-oxidation\nRemoving amino nitrogen connects glutamate with the central carbon cycle.\nThe GLUD1 study identifies glutamate oxidation to 2-oxoglutarate as the enzyme reaction underlying its metabolic and regulatory investigation.\nModel: Established reaction described in a primary human GLUD1 genetic/functional study.\nLimitations: Reaction background is distinguished from the directly measured mutant regulation; this record does not quantify tissue flux.\nEvidence access: Primary abstract\nHyperinsulinism and hyperammonemia in infants with regulatory mutations of the glutamate dehydrogenase gene. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9571255/ · DOI 10.1056/NEJM199805073381904","model_system":"Established reaction described in a primary human GLUD1 genetic/functional study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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