{"id":"1da4534b-5941-5947-a6b3-79be68c62460","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-b6-secretion-null","predicate":"did_not_increase","statement":"Evening pyridoxine did not significantly change melatonin onset, peak, mean or AUC relative to placebo.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"acdee2ec-0e2c-5a43-81c3-95337e88016a","mechanism_event_label":"An enzyme needs B6, but giving extra B6 did not increase the hormone in this experiment.","subject":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"object":{"id":"3e333fd8-10c9-57be-b97e-af220809ee2b","slug":"nocturnal-melatonin-concentration","display_name":"Nocturnal circulating melatonin concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"acdee2ec-0e2c-5a43-81c3-95337e88016a","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-b6-secretion-null-event","event_type":"observed_intervention","label":"An enzyme needs B6, but giving extra B6 did not increase the hormone in this experiment.","description":"Evening pyridoxine did not significantly change melatonin onset, peak, mean or AUC relative to placebo.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3e333fd8-10c9-57be-b97e-af220809ee2b","slug":"nocturnal-melatonin-concentration","display_name":"Nocturnal circulating melatonin concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/melatonin-research/12080281.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"04727c17cd3152a4749ec25ff15b36b827efb885501ce058d436fbbea1e9e7a9\", \"start_char\": 0, \"end_char\": 1222, \"text_sha256\": \"04727c17cd3152a4749ec25ff15b36b827efb885501ce058d436fbbea1e9e7a9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Placebo-controlled evening pyridoxine experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 mg pyridoxine at 17:00; evening/night sampling","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Acute exposure in healthy men. Null supplementation response does not negate the enzymatic requirement for PLP or settle effects in B6 deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Melatonin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Twelve healthy men","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An enzyme needs B6, but giving extra B6 did not increase the hormone in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[melatonin-p12080281] The effect of pyridoxine administration on melatonin secretion in normal men. (2002). https://pubmed.ncbi.nlm.nih.gov/12080281/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Melatonin secretion, temperature and polysomnography","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8325ff96-d979-528b-9288-3c20bc379fef","evidence_kind":"source_excerpt","locator":"Lines 773-784","start_line":773,"end_line":784,"excerpt":"### melatonin-b6-secretion-null\nEvening pyridoxine did not significantly change melatonin onset, peak, mean or AUC relative to placebo.\nCondition category: normal\nnutrient_topic: Melatonin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An enzyme needs B6, but giving extra B6 did not increase the hormone in this experiment.\norganism: Twelve healthy men\ntissue_or_cell_type: Melatonin secretion, temperature and polysomnography\nexperimental_model: Placebo-controlled evening pyridoxine experiment\nlimitations: Acute exposure in healthy men. Null supplementation response does not negate the enzymatic requirement for PLP or settle effects in B6 deficiency.\nexposure: 100 mg pyridoxine at 17:00; evening/night sampling\nevidence_span: {\"source_cache\": \"artifacts/melatonin-research/12080281.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"04727c17cd3152a4749ec25ff15b36b827efb885501ce058d436fbbea1e9e7a9\", \"start_char\": 0, \"end_char\": 1222, \"text_sha256\": \"04727c17cd3152a4749ec25ff15b36b827efb885501ce058d436fbbea1e9e7a9\"}\n[melatonin-p12080281] The effect of pyridoxine administration on melatonin secretion in normal men. (2002). https://pubmed.ncbi.nlm.nih.gov/12080281/","model_system":"Placebo-controlled evening pyridoxine experiment","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [melatonin-p12080281] The effect of pyridoxine administration on melatonin secretion in normal men. (2002). https://pubmed.ncbi.nlm.nih.gov/12080281/","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bab588de-d217-5038-9c8b-b41222eba08b","stable_key":"import-3279ff78-2460-5064-bfc0-3f2987af85a0","title":"Melatonin: synthesis, receptors, circadian timing and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ff4f279d263f95a69da8e7e8a6c6318ab6ba1b55254dbbda1bfa9ce437e1db80","revision_id":"0318eef8-ff38-513e-8ffa-458146ca5e77","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}