{"id":"1d73e5a8-35c4-5d0a-9150-2bd3db86f4e9","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-creatine-specificity","predicate":"does_not_phosphorylate_in_assay","statement":"Four purified human creatine-kinase isoforms phosphorylated creatine but not taurocyamine, guanidinobutyrate or guanidinopropionate at 10 mM test substrate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"c54d70c9-987e-547b-a083-50de8805adac","mechanism_event_label":"Related guanidino compounds are not automatically interchangeable energy buffers.","subject":{"id":"a72237a3-5780-5caa-8c9e-89d6cea0900b","slug":"human-creatine-kinase-substrate-panel","display_name":"Human CKB, CKM, CKMT1 and CKMT2 substrate panel","entity_type_key":"cellular_process"},"object":{"id":"8a2c2166-001d-5945-ac81-a303d5795133","slug":"taurocyamine","display_name":"Taurocyamine / guanidinotaurine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c54d70c9-987e-547b-a083-50de8805adac","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-creatine-specificity-event","event_type":"observed_relationship","label":"Related guanidino compounds are not automatically interchangeable energy buffers.","description":"Four purified human creatine-kinase isoforms phosphorylated creatine but not taurocyamine, guanidinobutyrate or guanidinopropionate at 10 mM test substrate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a72237a3-5780-5caa-8c9e-89d6cea0900b","slug":"human-creatine-kinase-substrate-panel","display_name":"Human CKB, CKM, CKMT1 and CKMT2 substrate panel","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8a2c2166-001d-5945-ac81-a303d5795133","slug":"taurocyamine","display_name":"Taurocyamine / guanidinotaurine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"508e25ee-c4f9-53ac-9b9b-3b122a90c6d9","slug":"4-guanidinobutyrate","display_name":"4-Guanidinobutyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""},{"entity":{"id":"e6d084fe-fe16-527d-a5cf-375696c81b24","slug":"3-guanidinopropionate","display_name":"3-Guanidinopropionate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":7,"notes":""},{"entity":{"id":"38918f7a-1ee6-547a-acc6-7182435a8a6d","slug":"ckb","display_name":"Human creatine kinase B / CKB","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":8,"notes":""},{"entity":{"id":"8592e998-bd0e-5b78-bf1d-7658834ff0e2","slug":"human-ckm","display_name":"Human muscle creatine kinase / CKM","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":9,"notes":""},{"entity":{"id":"6a61b4fe-179f-5919-bedf-647ebcd3c176","slug":"human-ckmt1","display_name":"Human ubiquitous mitochondrial creatine kinase / CKMT1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":10,"notes":""},{"entity":{"id":"19b467f0-470e-5a93-92cc-cfbeab1d5c65","slug":"human-ckmt2","display_name":"Human sarcomeric mitochondrial creatine kinase / CKMT2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":11,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; Figure 4A","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human recombinant kinases; radiolabeled ATP and TLC; annelid taurocyamine kinase positive control.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The experimental result does not exclude every reaction at other conditions, but does not support replacing creatine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Related guanidino compounds are not automatically interchangeable energy buffers.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Guanidino acid hydrolysis by the human enzyme annotated as agmatinase. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36543883/ · DOI 10.1038/s41598-022-26655-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ccfba5e7-4960-5ebf-a7da-904b20c0b8cf","evidence_kind":"source_excerpt","locator":"Lines 212-218","start_line":212,"end_line":218,"excerpt":"## agmatine-sulfate-creatine-specificity\nRelated guanidino compounds are not automatically interchangeable energy buffers.\nFour purified human creatine-kinase isoforms phosphorylated creatine but not taurocyamine, guanidinobutyrate or guanidinopropionate at 10 mM test substrate.\nModel: Human recombinant kinases; radiolabeled ATP and TLC; annelid taurocyamine kinase positive control.\nLimitations: The experimental result does not exclude every reaction at other conditions, but does not support replacing creatine.\nEvidence access: Primary full text; Figure 4A\nGuanidino acid hydrolysis by the human enzyme annotated as agmatinase. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36543883/ · DOI 10.1038/s41598-022-26655-4","model_system":"Human recombinant kinases; radiolabeled ATP and TLC; annelid taurocyamine kinase positive control.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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