{"id":"1d23e0d2-b059-58c4-9175-d2089f843494","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-dnmt","predicate":"inhibits","statement":"EGCG competitively inhibited DNMT activity in nuclear extracts, Ki 6.89 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c5787924-b376-56e9-8055-6c75559f7bd1","mechanism_event_label":"EGCG affected a DNA-methylation assay.","subject":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"object":{"id":"4d5e6ed1-5e0e-5039-b388-53e8efcfaa31","slug":"dnmt-family","display_name":"DNA cytosine methyltransferase family","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"c5787924-b376-56e9-8055-6c75559f7bd1","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-dnmt-event","event_type":"observed_relationship","label":"EGCG affected a DNA-methylation assay.","description":"EGCG competitively inhibited DNMT activity in nuclear extracts, Ki 6.89 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4d5e6ed1-5e0e-5039-b388-53e8efcfaa31","slug":"dnmt-family","display_name":"DNA cytosine methyltransferase family","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Nuclear-extract enzyme assay; molecular docking was also reported.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The extract assay is not purified DNMT1-specific proof; modeled binding contacts remain predictions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"EGCG affected a DNA-methylation assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Tea polyphenol (-)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines. · 2003 · https://pubmed.ncbi.nlm.nih.gov/14633667/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"00c489d3-8fed-5ef3-bcb1-c3349a5c5f5b","evidence_kind":"source_excerpt","locator":"Lines 252-258","start_line":252,"end_line":258,"excerpt":"## egcg-dnmt\nEGCG affected a DNA-methylation assay.\nEGCG competitively inhibited DNMT activity in nuclear extracts, Ki 6.89 micromolar.\nModel: Nuclear-extract enzyme assay; molecular docking was also reported.\nLimitations: The extract assay is not purified DNMT1-specific proof; modeled binding contacts remain predictions.\nEvidence access: primary abstract.\nTea polyphenol (-)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines. · 2003 · https://pubmed.ncbi.nlm.nih.gov/14633667/","model_system":"Nuclear-extract enzyme assay; molecular docking was also reported.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b101c8d4d96ee03ff97eb1922c7bb0f96943295af473fdc3ef98b0dbd62fe8c7","revision_id":"e19bc5b2-ee3c-5f67-b5c5-9dc3b7d5f850","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}