{"id":"1d1b7487-c9c1-5f9a-9772-7b6371a30855","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-acda-loss","predicate":"required_in_reductive_route","statement":"C. sporogenes acdA disruption impaired the reductive aromatic-amino-acid pathway leading to indolepropionate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"244bc94f-a1b6-54d2-8dc9-e217f6132448","mechanism_event_label":"A later reduction step is another potential production bottleneck.","subject":{"id":"6b3c6597-7e91-5384-82f2-6ab63c2acc14","slug":"c-sporogenes-acda","display_name":"Clostridium sporogenes AcdA","entity_type_key":"protein"},"object":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"244bc94f-a1b6-54d2-8dc9-e217f6132448","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-acda-loss-event","event_type":"observed_relationship","label":"A later reduction step is another potential production bottleneck.","description":"C. sporogenes acdA disruption impaired the reductive aromatic-amino-acid pathway leading to indolepropionate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b3c6597-7e91-5384-82f2-6ab63c2acc14","slug":"c-sporogenes-acda","display_name":"Clostridium sporogenes AcdA","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text, Figure 3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bacterial targeted-mutant metabolomics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not equate a host ACAD gene with this microbial enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A later reduction step is another potential production bottleneck.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29168502/ · DOI 10.1038/nature24661","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"a4a4700d-77b6-5b91-9cc1-824ab458adad","evidence_kind":"source_excerpt","locator":"Lines 506-512","start_line":506,"end_line":512,"excerpt":"## tryptophan-acda-loss\nA later reduction step is another potential production bottleneck.\nC. sporogenes acdA disruption impaired the reductive aromatic-amino-acid pathway leading to indolepropionate.\nModel: Bacterial targeted-mutant metabolomics.\nLimitations: Do not equate a host ACAD gene with this microbial enzyme.\nEvidence access: Primary full text, Figure 3\nA gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29168502/ · DOI 10.1038/nature24661","model_system":"Bacterial targeted-mutant metabolomics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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