{"id":"1d06f654-dfc1-536e-ac61-ed8aa4dd0f1c","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-neuronal-transport","predicate":"supports","statement":"Rat neuronal uptake showed a sodium-independent component consistent with CAT1, with Km 47 ± 8 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b9839848-46c7-5f9a-a5b1-999ffc5d4272","mechanism_event_label":"Neurons used a different transporter pattern from stimulated glial cells.","subject":{"id":"425c5ca9-452f-5dfd-9ebf-f6d5c2f9a1a4","slug":"rat-slc7a1","display_name":"Rat cationic amino acid transporter 1 / Slc7a1","entity_type_key":"protein"},"object":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b9839848-46c7-5f9a-a5b1-999ffc5d4272","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-neuronal-transport-event","event_type":"observed_relationship","label":"Neurons used a different transporter pattern from stimulated glial cells.","description":"Rat neuronal uptake showed a sodium-independent component consistent with CAT1, with Km 47 ± 8 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"425c5ca9-452f-5dfd-9ebf-f6d5c2f9a1a4","slug":"rat-slc7a1","display_name":"Rat cationic amino acid transporter 1 / Slc7a1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary rat hypothalamus/brainstem cultures; uptake and transcript assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"CAT2 transcripts were not detected; the kinetic result does not establish a human blood threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Neurons used a different transporter pattern from stimulated glial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Membrane transport of neuronal nitric oxide synthase substrate L-arginine is constitutively expressed with CAT1 and 4F2hc, but not CAT2 or rBAT. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9681446/ · DOI 10.1046/j.1471-4159.1998.71020564.x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cd4a252c-6972-5438-9ee1-e38f5f2785ee","evidence_kind":"source_excerpt","locator":"Lines 62-68","start_line":62,"end_line":68,"excerpt":"## arg-neuronal-transport\nNeurons used a different transporter pattern from stimulated glial cells.\nRat neuronal uptake showed a sodium-independent component consistent with CAT1, with Km 47 ± 8 micromolar.\nModel: Primary rat hypothalamus/brainstem cultures; uptake and transcript assays.\nLimitations: CAT2 transcripts were not detected; the kinetic result does not establish a human blood threshold.\nEvidence access: Primary abstract\nMembrane transport of neuronal nitric oxide synthase substrate L-arginine is constitutively expressed with CAT1 and 4F2hc, but not CAT2 or rBAT. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9681446/ · DOI 10.1046/j.1471-4159.1998.71020564.x","model_system":"Primary rat hypothalamus/brainstem cultures; uptake and transcript assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"ec0e9a3fb72b7485534e4372a074ac7624a399ff962e55690eb462217b5149ec","revision_id":"af027270-5535-51ae-85ff-9c76c518c962","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}