{"id":"1d04b58f-baa0-51b3-b9d7-654e5192a172","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:embryonic-glycine-formate","predicate":"supplies-carbon-to","statement":"Isolated mouse embryonic mitochondria converted labeled glycine carbon into formate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a81d4907-029b-5dd2-a83b-41e077c7897f","mechanism_event_label":"Glycine cleavage can feed folate-bound carbon into mitochondrial formate production.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a81d4907-029b-5dd2-a83b-41e077c7897f","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:embryonic-glycine-formate-event","event_type":"biochemical_relationship","label":"Glycine cleavage can feed folate-bound carbon into mitochondrial formate production.","description":"Isolated mouse embryonic mitochondria converted labeled glycine carbon into formate.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"206d41a9-3025-5cd2-8fa0-ccf9563d501a","slug":"glycine-cleavage-flux","display_name":"Glycine cleavage flux","entity_type_key":"cellular_process"},"role":"upstream pathway","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"role":"one-carbon carrier","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Handoff to existing PLP-dependent glycine decarboxylase and lipoyl-GCSH/AMT folate transfer evidence; not new deficiency evidence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radiolabeled mitochondrial flux assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No B6 or lipoate dietary perturbation tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Glycine cleavage can feed folate-bound carbon into mitochondrial formate production.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[pike-2010] Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos (2010). https://pubmed.ncbi.nlm.nih.gov/19948730/ DOI: 10.1074/jbc.m109.079855","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Embryonic mitochondria","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6462f944-fbf3-5aba-82a2-a79628108885","evidence_kind":"source_excerpt","locator":"Lines 1096-1107","start_line":1096,"end_line":1107,"excerpt":"### embryonic-glycine-formate\nIsolated mouse embryonic mitochondria converted labeled glycine carbon into formate.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Glycine cleavage can feed folate-bound carbon into mitochondrial formate production.\norganism: Mus musculus\ntissue_or_cell_type: Embryonic mitochondria\nexperimental_model: Radiolabeled mitochondrial flux assay\nlimitations: No B6 or lipoate dietary perturbation tested.\nexposure: Assay conditions described in the linked primary study.\ncross_nutrient: Handoff to existing PLP-dependent glycine decarboxylase and lipoyl-GCSH/AMT folate transfer evidence; not new deficiency evidence.\n[pike-2010] Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos (2010). https://pubmed.ncbi.nlm.nih.gov/19948730/ DOI: 10.1074/jbc.m109.079855","model_system":"Radiolabeled mitochondrial flux assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [pike-2010] Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos (2010). https://pubmed.ncbi.nlm.nih.gov/19948730/ DOI: 10.1074/jbc.m109.079855","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}