{"id":"1cdcfae9-621f-5fdd-b3a8-dba54afaf568","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-cyp4f2-gamma-hydroxylation","predicate":"catalyzes_formation","statement":"Recombinant human CYP4F2 catalyzed terminal side-chain hydroxylation of gamma-tocopherol to its 13′-hydroxy product.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2ef0cd16-dcc1-5637-b3dc-51024145a08c","mechanism_event_label":"CYP4F2 begins breakdown of this tocopherol form.","subject":{"id":"0510331f-d4bd-57ba-97a0-ce64171ec3e7","slug":"cyp4f2","display_name":"Human cytochrome P450 4F2","entity_type_key":"protein"},"object":{"id":"7fac36ce-6afb-59ca-9694-865c0b257f01","slug":"13-prime-hydroxy-gamma-tocopherol","display_name":"13′-Hydroxy-gamma-tocopherol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"2ef0cd16-dcc1-5637-b3dc-51024145a08c","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-cyp4f2-gamma-hydroxylation-event","event_type":"biochemical_relationship","label":"CYP4F2 begins breakdown of this tocopherol form.","description":"Recombinant human CYP4F2 catalyzed terminal side-chain hydroxylation of gamma-tocopherol to its 13′-hydroxy product.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0510331f-d4bd-57ba-97a0-ce64171ec3e7","slug":"cyp4f2","display_name":"Human cytochrome P450 4F2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7fac36ce-6afb-59ca-9694-865c0b257f01","slug":"13-prime-hydroxy-gamma-tocopherol","display_name":"13′-Hydroxy-gamma-tocopherol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2de3a4d0-56b1-57f9-9e9d-934ba52a1753","slug":"gamma-tocopherol","display_name":"Gamma-tocopherol","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"reducing_cofactor","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human P450 enzyme comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"gamma-Tocopherol substrate with NADPH; quantitative incubation details not assigned here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Initial oxidation only; other enzymes perform subsequent side-chain shortening.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human protein in recombinant microsomes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CYP4F2 begins breakdown of this tocopherol form.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sontag2002] Cytochrome P450 omega-hydroxylase pathway of tocopherol catabolism. Novel mechanism of regulation of vitamin E status. (2002). https://pubmed.ncbi.nlm.nih.gov/11997390/ DOI: 10.1074/jbc.m201466200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Microsomal enzyme preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c9409c10-b507-5cc2-88a3-3bb1626b7e60","evidence_kind":"source_excerpt","locator":"Lines 415-426","start_line":415,"end_line":426,"excerpt":"### ve-transport-cyp4f2-gamma-hydroxylation\nRecombinant human CYP4F2 catalyzed terminal side-chain hydroxylation of gamma-tocopherol to its 13′-hydroxy product.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CYP4F2 begins breakdown of this tocopherol form.\norganism: Human protein in recombinant microsomes\ntissue_or_cell_type: Microsomal enzyme preparation\nexperimental_model: Recombinant human P450 enzyme comparison\nlimitations: Initial oxidation only; other enzymes perform subsequent side-chain shortening.\nexposure: gamma-Tocopherol substrate with NADPH; quantitative incubation details not assigned here.\ncross_nutrient: false\n[sontag2002] Cytochrome P450 omega-hydroxylase pathway of tocopherol catabolism. 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