{"id":"1cc2ad82-1a78-5a27-8787-582cf8e77f55","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-pc-communication","predicate":"enables_catalytic_coordination_of","statement":"Acetyl-CoA-dependent stabilization of human PC triggered ATP hydrolysis and communication between its two reaction centers.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9baa0386-1e70-596c-a54d-347cba3e4d52","mechanism_event_label":"The biotin-carrying enzyme coordinates two reaction stations rather than performing one isolated chemical step.","subject":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"object":{"id":"b84a1471-7246-543a-8f35-2bedbca6bf0b","slug":"pc","display_name":"Human pyruvate carboxylase / PC","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"9baa0386-1e70-596c-a54d-347cba3e4d52","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-pc-communication-event","event_type":"biochemical_relationship","label":"The biotin-carrying enzyme coordinates two reaction stations rather than performing one isolated chemical step.","description":"Acetyl-CoA-dependent stabilization of human PC triggered ATP hydrolysis and communication between its two reaction centers.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"hydrolyzed substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"carboxyl carrier","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b84a1471-7246-543a-8f35-2bedbca6bf0b","slug":"pc","display_name":"Human pyruvate carboxylase / PC","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/36283412.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2a5fb8ec1c27e243b8c8ba158f2c58ab1592d9895eecf16438175b2a5778de15\", \"start_char\": 0, \"end_char\": 1060, \"text_sha256\": \"2a5fb8ec1c27e243b8c8ba158f2c58ab1592d9895eecf16438175b2a5778de15\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Time-resolved cryo-EM and biochemical analysis of human PC","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Pyruvate, ATP and acetyl-CoA catalytic conditions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme regulation is not evidence that consuming B5 or biotin stimulates this pathway in every tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The biotin-carrying enzyme coordinates two reaction stations rather than performing one isolated chemical step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p36283412] Mechanistic insight into allosteric activation of human pyruvate carboxylase by acetyl-CoA. (2022). https://pubmed.ncbi.nlm.nih.gov/36283412/ DOI: 10.1016/j.molcel.2022.09.033","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified human pyruvate carboxylase","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"723f7f3c-abb7-5f9e-8897-14feef490fe6","evidence_kind":"source_excerpt","locator":"Lines 572-583","start_line":572,"end_line":583,"excerpt":"### b7-pc-communication\nAcetyl-CoA-dependent stabilization of human PC triggered ATP hydrolysis and communication between its two reaction centers.\nCondition category: normal\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The biotin-carrying enzyme coordinates two reaction stations rather than performing one isolated chemical step.\norganism: Homo sapiens\ntissue_or_cell_type: Purified human pyruvate carboxylase\nexperimental_model: Time-resolved cryo-EM and biochemical analysis of human PC\nlimitations: Purified-enzyme regulation is not evidence that consuming B5 or biotin stimulates this pathway in every tissue.\nexposure: Pyruvate, ATP and acetyl-CoA catalytic conditions\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/36283412.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2a5fb8ec1c27e243b8c8ba158f2c58ab1592d9895eecf16438175b2a5778de15\", \"start_char\": 0, \"end_char\": 1060, \"text_sha256\": \"2a5fb8ec1c27e243b8c8ba158f2c58ab1592d9895eecf16438175b2a5778de15\"}\n[b7-p36283412] Mechanistic insight into allosteric activation of human pyruvate carboxylase by acetyl-CoA. 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