{"id":"1cbfd24b-e061-5328-a121-d38ffac08c50","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-fungal-chitin","predicate":"increases_chdfa_susceptibility","statement":"Chitin-synthesis inhibition with nikkomycin Z increased Candida susceptibility to CHD-FA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7a5f60f7-b4b4-51a2-aaa4-6d6a034e8a82","mechanism_event_label":"Weakening the wall made the tested membrane-active exposure more effective.","subject":{"id":"0901383d-50b1-5332-82a8-89e391a3122c","slug":"nikkomycin-z","display_name":"Nikkomycin Z","entity_type_key":"drug"},"object":{"id":"27f39d28-d3c0-59cb-a7f3-8bd3afca91a5","slug":"candida-chdfa-susceptibility","display_name":"Candida susceptibility to CHD-FA","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7a5f60f7-b4b4-51a2-aaa4-6d6a034e8a82","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-fungal-chitin-event","event_type":"observed_relationship","label":"Weakening the wall made the tested membrane-active exposure more effective.","description":"Chitin-synthesis inhibition with nikkomycin Z increased Candida susceptibility to CHD-FA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0901383d-50b1-5332-82a8-89e391a3122c","slug":"nikkomycin-z","display_name":"Nikkomycin Z","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"27f39d28-d3c0-59cb-a7f3-8bd3afca91a5","slug":"candida-chdfa-susceptibility","display_name":"Candida susceptibility to CHD-FA","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5184fd06-5a8e-5e9d-99ec-c2feaa59ccde","slug":"carbohydrate-derived-fulvic-acid","display_name":"Carbohydrate-derived fulvic acid / CHD-FA (preparation class)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Candida planktonic and biofilm combination experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This supports a cell-envelope mechanism; it is not a clinically established combination.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Weakening the wall made the tested membrane-active exposure more effective.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carbohydrate Derived Fulvic Acid: An in vitro Investigation of a Novel Membrane Active Antiseptic Agent Against Candida albicans Biofilms. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22479260/ · DOI 10.3389/fmicb.2012.00116","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8a449d21-46f2-53f2-b5b8-3b264ebe8148","evidence_kind":"source_excerpt","locator":"Lines 140-146","start_line":140,"end_line":146,"excerpt":"## fulvic-acid-fungal-chitin\nWeakening the wall made the tested membrane-active exposure more effective.\nChitin-synthesis inhibition with nikkomycin Z increased Candida susceptibility to CHD-FA.\nModel: Candida planktonic and biofilm combination experiments.\nLimitations: This supports a cell-envelope mechanism; it is not a clinically established combination.\nEvidence access: Primary full text\nCarbohydrate Derived Fulvic Acid: An in vitro Investigation of a Novel Membrane Active Antiseptic Agent Against Candida albicans Biofilms. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22479260/ · DOI 10.3389/fmicb.2012.00116","model_system":"Candida planktonic and biofilm combination experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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