{"id":"1c3b06d0-1a29-5a37-b7f5-74433e9e6f48","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:aadat-transamination","predicate":"converted_to","statement":"PLP-dependent AADAT transfers the amino group from aminoadipate to 2-oxoglutarate, generating 2-oxoadipate and glutamate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3e8f0629-a2c0-501a-baf4-9ddebf97a425","mechanism_event_label":"AADAT removes the remaining amino group.","subject":{"id":"82144983-75c1-572a-a5f3-88d6e2a99775","slug":"alpha-aminoadipate","display_name":"L-alpha-Aminoadipate","entity_type_key":"small_molecule"},"object":{"id":"22cbb32f-fa6b-57a0-a02d-8f2083585223","slug":"2-oxoadipate","display_name":"2-Oxoadipate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"3e8f0629-a2c0-501a-baf4-9ddebf97a425","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:aadat-transamination-event","event_type":"biochemical_relationship","label":"AADAT removes the remaining amino group.","description":"PLP-dependent AADAT transfers the amino group from aminoadipate to 2-oxoglutarate, generating 2-oxoadipate and glutamate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b0d4ed40-858b-50e8-acbb-669452624d93","slug":"aadat","display_name":"AADAT / KAT II","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"82144983-75c1-572a-a5f3-88d6e2a99775","slug":"alpha-aminoadipate","display_name":"L-alpha-Aminoadipate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"amino_acceptor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"22cbb32f-fa6b-57a0-a02d-8f2083585223","slug":"2-oxoadipate","display_name":"2-Oxoadipate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human cDNA and recombinant bacterial expression; Purified recombinant human enzyme; kinetics and crystallography","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"AADAT also accepts other substrates; substrate breadth is not lysine-specific regulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"AADAT removes the remaining amino group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[goh2002] Characterization of the human gene encoding alpha-aminoadipate aminotransferase (AADAT). (2002). https://pubmed.ncbi.nlm.nih.gov/12126930/ DOI: 10.1016/S1096-7192(02)00037-9\n[han2008] Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II (2008). https://pmc.ncbi.nlm.nih.gov/articles/PMC2559858/ DOI: 10.1042/BSR20080085","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial lysine catabolism; human expression highest in liver in cloning study","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a5bc2e18-6aa4-57ee-8024-73a3d2846aa9","evidence_kind":"source_excerpt","locator":"Lines 114-123","start_line":114,"end_line":123,"excerpt":"### aadat-transamination\nPLP-dependent AADAT transfers the amino group from aminoadipate to 2-oxoglutarate, generating 2-oxoadipate and glutamate.\nPlain language: AADAT removes the remaining amino group.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Mitochondrial lysine catabolism; human expression highest in liver in cloning study\nexperimental_model: Human cDNA and recombinant bacterial expression; Purified recombinant human enzyme; kinetics and crystallography\nlimitations: AADAT also accepts other substrates; substrate breadth is not lysine-specific regulation.\n[goh2002] Characterization of the human gene encoding alpha-aminoadipate aminotransferase (AADAT). (2002). https://pubmed.ncbi.nlm.nih.gov/12126930/ DOI: 10.1016/S1096-7192(02)00037-9\n[han2008] Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II (2008). https://pmc.ncbi.nlm.nih.gov/articles/PMC2559858/ DOI: 10.1042/BSR20080085","model_system":"Human cDNA and recombinant bacterial expression; Purified recombinant human enzyme; kinetics and crystallography","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [goh2002] Characterization of the human gene encoding alpha-aminoadipate aminotransferase (AADAT). (2002). https://pubmed.ncbi.nlm.nih.gov/12126930/ DOI: 10.1016/S1096-7192(02)00037-9; [han2008] Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II (2008). https://pmc.ncbi.nlm.nih.gov/articles/PMC2559858/ DOI: 10.1042/BSR20080085","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}