{"id":"1c144a9f-4ebc-534e-8ede-94b02e109390","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-hela-depolarization","predicate":"increases","statement":"Coumarin dose-dependently reduced HeLa mitochondrial membrane potential.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a7be4b0d-4e82-52ff-b39d-966d291ed376","mechanism_event_label":"The mitochondrial electrical gradient weakened.","subject":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"object":{"id":"68d21241-0626-5527-9636-46e1a577b470","slug":"hela-mitochondrial-depolarization","display_name":"Mitochondrial depolarization in HeLa cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a7be4b0d-4e82-52ff-b39d-966d291ed376","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-hela-depolarization-event","event_type":"biochemical_relationship","label":"The mitochondrial electrical gradient weakened.","description":"Coumarin dose-dependently reduced HeLa mitochondrial membrane potential.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"68d21241-0626-5527-9636-46e1a577b470","slug":"hela-mitochondrial-depolarization","display_name":"Mitochondrial depolarization in HeLa cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coumarin-research/18210747.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\", \"start_char\": 0, \"end_char\": 1237, \"text_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell viability, cell cycle, protein and mitochondrial assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Coumarin; reported viability IC50 54.2 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cultured cancer-cell response does not establish treatment efficacy; indexed abstract does not specify all exposure durations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coumarin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The mitochondrial electrical gradient weakened.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coumarin-p18210747] Coumarin induces cell cycle arrest and apoptosis in human cervical cancer HeLa cells through a mitochondria- and caspase-3 dependent mechanism and NF-kappaB down-regulation. 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