{"id":"1bd86194-0fca-50a2-b517-11f938731c50","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-hrg1-recycling","predicate":"mediates","statement":"Hrg1 localized to phagolysosomal membranes during erythrophagocytosis and transported heme toward the macrophage cytoplasm.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"439a7c00-1761-5b29-95e5-1b7a262e7f56","mechanism_event_label":"Macrophages must move heme out of the compartment where old red cells are broken down.","subject":{"id":"ec3024bf-ca6b-542e-908c-273a80ea8221","slug":"mouse-slc48a1","display_name":"Mouse heme transporter HRG1 / Slc48a1","entity_type_key":"protein"},"object":{"id":"abb1275a-6858-5929-bc64-1eecf6eda2c1","slug":"phagolysosomal-heme-export","display_name":"Heme export from the macrophage phagolysosome","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"439a7c00-1761-5b29-95e5-1b7a262e7f56","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-hrg1-recycling-event","event_type":"biochemical_relationship","label":"Macrophages must move heme out of the compartment where old red cells are broken down.","description":"Hrg1 localized to phagolysosomal membranes during erythrophagocytosis and transported heme toward the macrophage cytoplasm.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2e1f7e0a-8b54-5ea3-9d34-01e7167f9097","slug":"heme","display_name":"Heme","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ec3024bf-ca6b-542e-908c-273a80ea8221","slug":"mouse-slc48a1","display_name":"Mouse heme transporter HRG1 / Slc48a1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"abb1275a-6858-5929-bc64-1eecf6eda2c1","slug":"phagolysosomal-heme-export","display_name":"Heme export from the macrophage phagolysosome","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iron-research/23395172.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9ffc7159cfb418fed987d6f3f9e34829861a8f4ede0fe564a2714ef0bc96968b\", \"start_char\": 0, \"end_char\": 1074, \"text_sha256\": \"9ffc7159cfb418fed987d6f3f9e34829861a8f4ede0fe564a2714ef0bc96968b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Macrophage depletion, localization and human variant assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Hrg1 depletion and missense variants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Recycling pathway experiment; no estimate of dietary iron requirements is derived.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse macrophages and human HRG1 variants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Macrophages must move heme out of the compartment where old red cells are broken down.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iron-p23395172] HRG1 is essential for heme transport from the phagolysosome of macrophages during erythrophagocytosis. (2013). https://pubmed.ncbi.nlm.nih.gov/23395172/ DOI: 10.1016/j.cmet.2013.01.005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Phagolysosomes during erythrophagocytosis","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ec90250c-2a0a-5708-b1c3-a674e3c84465","evidence_kind":"source_excerpt","locator":"Lines 550-561","start_line":550,"end_line":561,"excerpt":"### iron-hrg1-recycling\nHrg1 localized to phagolysosomal membranes during erythrophagocytosis and transported heme toward the macrophage cytoplasm.\nCondition category: normal\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Macrophages must move heme out of the compartment where old red cells are broken down.\norganism: Mouse macrophages and human HRG1 variants\ntissue_or_cell_type: Phagolysosomes during erythrophagocytosis\nexperimental_model: Macrophage depletion, localization and human variant assays\nlimitations: Recycling pathway experiment; no estimate of dietary iron requirements is derived.\nexposure: Hrg1 depletion and missense variants\nevidence_span: {\"source_cache\": \"artifacts/iron-research/23395172.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9ffc7159cfb418fed987d6f3f9e34829861a8f4ede0fe564a2714ef0bc96968b\", \"start_char\": 0, \"end_char\": 1074, \"text_sha256\": \"9ffc7159cfb418fed987d6f3f9e34829861a8f4ede0fe564a2714ef0bc96968b\"}\n[iron-p23395172] HRG1 is essential for heme transport from the phagolysosome of macrophages during erythrophagocytosis. 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