{"id":"1bceef18-a203-5331-be7a-12a40d85392e","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-isoniazid-pl-conjugation","predicate":"reacts-with","statement":"Isoniazid and pyridoxal formed PIH without cells or enzymes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b4ee6dce-8132-55e2-bbd8-3ddd410e9a1c","mechanism_event_label":"A drug can chemically capture a B6 vitamer.","subject":{"id":"3a6f1b6d-7c5c-573a-8f73-5f96d1967baa","slug":"isoniazid","display_name":"Isoniazid","entity_type_key":"small_molecule"},"object":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b4ee6dce-8132-55e2-bbd8-3ddd410e9a1c","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-isoniazid-pl-conjugation-event","event_type":"biochemical_relationship","label":"A drug can chemically capture a B6 vitamer.","description":"Isoniazid and pyridoxal formed PIH without cells or enzymes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3a6f1b6d-7c5c-573a-8f73-5f96d1967baa","slug":"isoniazid","display_name":"Isoniazid","entity_type_key":"small_molecule"},"role":"reactant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"role":"reactant","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"af123b94-0b58-5894-83f0-9b8e681b8fe5","slug":"pyridoxal-isonicotinoyl-hydrazone","display_name":"Pyridoxal isonicotinoyl hydrazone","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Cell-free chemistry, primary human hepatocytes and HepG2/C3A cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"1 mM reactants in PBS; timed incubations at 4 or 37 C.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High-concentration cell-free chemistry; not proof of neuronal depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Not applicable; chemical reaction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A drug can chemically capture a B6 vitamer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[brewer-2019-isoniazid] The Isoniazid Metabolites Hydrazine and Pyridoxal Isonicotinoyl Hydrazone Modulate Heme Biosynthesis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6390808/ DOI: 10.1093/toxsci/kfy294","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free PBS","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9168a547-408f-57c7-9cf9-83ebaaed7521","evidence_kind":"source_excerpt","locator":"Lines 1271-1281","start_line":1271,"end_line":1281,"excerpt":"### b6-neuro-isoniazid-pl-conjugation\nIsoniazid and pyridoxal formed PIH without cells or enzymes.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A drug can chemically capture a B6 vitamer.\norganism: Not applicable; chemical reaction\ntissue_or_cell_type: Cell-free PBS\nexperimental_model: Cell-free chemistry, primary human hepatocytes and HepG2/C3A cells\nlimitations: High-concentration cell-free chemistry; not proof of neuronal depletion.\nexposure: 1 mM reactants in PBS; timed incubations at 4 or 37 C.\n[brewer-2019-isoniazid] The Isoniazid Metabolites Hydrazine and Pyridoxal Isonicotinoyl Hydrazone Modulate Heme Biosynthesis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6390808/ DOI: 10.1093/toxsci/kfy294","model_system":"Cell-free chemistry, primary human hepatocytes and HepG2/C3A cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [brewer-2019-isoniazid] The Isoniazid Metabolites Hydrazine and Pyridoxal Isonicotinoyl Hydrazone Modulate Heme Biosynthesis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6390808/ DOI: 10.1093/toxsci/kfy294","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}