{"id":"1b95f954-0135-507c-86c5-815fab67bd4a","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-rabbit-sodium-chloride","predicate":"cotransports","statement":"The cloned rabbit creatine transporter mediated sodium- and chloride-dependent creatine uptake, with an apparent Km of approximately 35 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4a862ba3-fd61-5430-b0bf-ee176cec8416","mechanism_event_label":"Creatine uptake used both sodium and chloride in this transport experiment.","subject":{"id":"c68e7125-e313-5a7b-a7bc-de17cb9f7bbb","slug":"rabbit-slc6a8","display_name":"Rabbit creatine transporter / Slc6a8","entity_type_key":"protein"},"object":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"4a862ba3-fd61-5430-b0bf-ee176cec8416","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-rabbit-sodium-chloride-event","event_type":"biochemical_relationship","label":"Creatine uptake used both sodium and chloride in this transport experiment.","description":"The cloned rabbit creatine transporter mediated sodium- and chloride-dependent creatine uptake, with an apparent Km of approximately 35 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"coupling_ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"coupling_ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c68e7125-e313-5a7b-a7bc-de17cb9f7bbb","slug":"rabbit-slc6a8","display_name":"Rabbit creatine transporter / Slc6a8","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/creatine-research/8473283.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"10a3e9e18054bc80e38fe289f59ba358853482082e69c87af0844ac90a6da5c7\", \"start_char\": 0, \"end_char\": 1330, \"text_sha256\": \"10a3e9e18054bc80e38fe289f59ba358853482082e69c87af0844ac90a6da5c7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rabbit transporter cloning and heterologous uptake assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Radiolabeled creatine uptake with ion dependence and substrate analogues","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rabbit expression assay; neither human ion stoichiometry nor a benefit from extra dietary salt is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Creatine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rabbit transporter expressed in COS-7 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Creatine uptake used both sodium and chloride in this transport experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[creatine-p8473283] A Na(+)-dependent creatine transporter in rabbit brain, muscle, heart, and kidney. cDNA cloning and functional expression. (1993). https://pubmed.ncbi.nlm.nih.gov/8473283/ DOI: 10.1016/s0021-9258(18)52891-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell plasma membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Sodium- and chloride-dependent creatine uptake with an apparent Km near 35 micromolar.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Sodium- and chloride-dependent creatine uptake with an apparent Km near 35 micromolar.","entity":null}],"evidence":[{"id":"9f411dce-6334-5246-a9cd-7381999b6776","evidence_kind":"source_excerpt","locator":"Lines 412-423","start_line":412,"end_line":423,"excerpt":"### creatine-rabbit-sodium-chloride\nThe cloned rabbit creatine transporter mediated sodium- and chloride-dependent creatine uptake, with an apparent Km of approximately 35 micromolar.\nCondition category: normal\nnutrient_topic: Creatine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Creatine uptake used both sodium and chloride in this transport experiment.\norganism: Rabbit transporter expressed in COS-7 cells\ntissue_or_cell_type: Cell plasma membrane\nexperimental_model: Rabbit transporter cloning and heterologous uptake assay\nlimitations: Rabbit expression assay; neither human ion stoichiometry nor a benefit from extra dietary salt is inferred.\nexposure: Radiolabeled creatine uptake with ion dependence and substrate analogues\nevidence_span: {\"source_cache\": \"artifacts/creatine-research/8473283.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"10a3e9e18054bc80e38fe289f59ba358853482082e69c87af0844ac90a6da5c7\", \"start_char\": 0, \"end_char\": 1330, \"text_sha256\": \"10a3e9e18054bc80e38fe289f59ba358853482082e69c87af0844ac90a6da5c7\"}\n[creatine-p8473283] A Na(+)-dependent creatine transporter in rabbit brain, muscle, heart, and kidney. cDNA cloning and functional expression. (1993). https://pubmed.ncbi.nlm.nih.gov/8473283/ DOI: 10.1016/s0021-9258(18)52891-x","model_system":"Rabbit transporter cloning and heterologous uptake assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [creatine-p8473283] A Na(+)-dependent creatine transporter in rabbit brain, muscle, heart, and kidney. cDNA cloning and functional expression. (1993). https://pubmed.ncbi.nlm.nih.gov/8473283/ DOI: 10.1016/s0021-9258(18)52891-x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"70358046-c5c3-5e9a-9fc9-eb01c3fff88b","stable_key":"import-44737fa3-b335-53f4-a644-b9878d4416ac","title":"Creatine: synthesis, transport, phosphocreatine energetics and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"548237d3e98a73a7dc02181807921fdd8b902adcee46248792258a761593eda9","revision_id":"88738255-8028-52fb-9c12-0fe92d8ce367","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}