{"id":"1b562a6a-190e-5205-82ac-ccf53e5b9b8d","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-human-proil1b-null","predicate":"no_detected_change_in_recorded_comparison","statement":"Short apigenin treatment after LPS priming did not decrease pro-IL-1β protein in the initial human-cell experiment.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f4cdadb2-22e1-5729-b730-e2160266abb4","mechanism_event_label":"Short apigenin treatment after LPS priming did not decrease pro-IL-1β protein in the initial human-cell experiment.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"e9fa1ab4-707e-5ff9-9d60-11f503b54c58","slug":"il1b","display_name":"Interleukin-1 beta / IL1B","entity_type_key":"cytokine"},"evidence_count":1,"mechanism_event":{"id":"f4cdadb2-22e1-5729-b730-e2160266abb4","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-human-proil1b-null-event","event_type":"experimental_observation","label":"Short apigenin treatment after LPS priming did not decrease pro-IL-1β protein in the initial human-cell experiment.","description":"### api-human-proil1b-null\n\nShort apigenin treatment after LPS priming did not decrease pro-IL-1β protein in the initial human-cell experiment.\nReduced release can occur without reduced precursor synthesis. TNF was unchanged in the initial experiment, although some later comparisons showed reductions.\nModel and exposure: Figure 1E–G; 100 micromolar, 30 minutes; immunoblot pro-protein.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | 2024 | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin after completed priming","sequence_order":0,"notes":""},{"entity":{"id":"e9fa1ab4-707e-5ff9-9d60-11f503b54c58","slug":"il1b","display_name":"Interleukin-1 beta / IL1B","entity_type_key":"cytokine"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"Apigenin after completed priming","comparator":"LPS priming alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"prior priming","comparator":"LPS priming alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"lipopolysaccharide","display_name":"Lipopolysaccharide","entity_type_key":"chemical_species"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin after completed priming\", \"comparator\": \"LPS priming alone\", \"endpoint\": \"Short apigenin treatment after LPS priming did not decrease pro-IL-1β protein in the initial human-cell experiment.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin after completed priming\"}, {\"entity_slug\": \"lipopolysaccharide\", \"state\": \"prior priming\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Figure 1E–G; 100 micromolar, 30 minutes; immunoblot pro-protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Short apigenin treatment after LPS priming did not decrease pro-IL-1β protein in the initial human-cell experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-human-proil1b-null; Reduced release can occur without reduced precursor synthesis. TNF was unchanged in the initial experiment, although some later comparisons showed reductions.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"73ed8c35-0bbc-52a8-a989-0d8e95436af6","evidence_kind":"source_excerpt","locator":"Lines 356-362","start_line":356,"end_line":362,"excerpt":"### api-human-proil1b-null\n\nShort apigenin treatment after LPS priming did not decrease pro-IL-1β protein in the initial human-cell experiment.\nReduced release can occur without reduced precursor synthesis. TNF was unchanged in the initial experiment, although some later comparisons showed reductions.\nModel and exposure: Figure 1E–G; 100 micromolar, 30 minutes; immunoblot pro-protein.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | 2024 | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Figure 1E–G; 100 micromolar, 30 minutes; immunoblot pro-protein.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}