{"id":"1b51badf-bdb7-5790-9a89-435ef925a634","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-sulfite-gssg-chemical","predicate":"reacts_in_cell_free_system_with","statement":"At 500 micromolar, sulfite increased measured GSH in a cell-free GSSG solution, consistent with disulfide-bond chemistry.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1fb71ffd-4581-5dfe-b143-991d06a5acf0","mechanism_event_label":"A chemical increase in measured GSH outside cells is different from restoring a living antioxidant system.","subject":{"id":"914ace7c-9176-53f7-80a9-9ee71d26ea52","slug":"sulfite","display_name":"Sulfite / SO3(2-)","entity_type_key":"ion"},"object":{"id":"1d9b9d7b-3fdf-57af-b9dd-00194fa1de9e","slug":"oxidized-glutathione","display_name":"GSSG","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"1fb71ffd-4581-5dfe-b143-991d06a5acf0","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-sulfite-gssg-chemical-event","event_type":"biochemical_relationship","label":"A chemical increase in measured GSH outside cells is different from restoring a living antioxidant system.","description":"At 500 micromolar, sulfite increased measured GSH in a cell-free GSSG solution, consistent with disulfide-bond chemistry.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"measured product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"914ace7c-9176-53f7-80a9-9ee71d26ea52","slug":"sulfite","display_name":"Sulfite / SO3(2-)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1d9b9d7b-3fdf-57af-b9dd-00194fa1de9e","slug":"oxidized-glutathione","display_name":"GSSG","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/25777939.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1768480178724867d4c7a98f723cfd1a0010f7973eae4a4baea3d1261a06fdd2\", \"start_char\": 0, \"end_char\": 2017, \"text_sha256\": \"1768480178724867d4c7a98f723cfd1a0010f7973eae4a4baea3d1261a06fdd2\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Sulfite/thiosulfate incubation of rat cortical slices and cell-free GSSG","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"One- versus three-hour incubations; 10 micromolar sulfite redox observations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Concentration, duration and preparation matter; GPx activity is not evidence of selenium depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus; cell-free comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A chemical increase in measured GSH outside cells is different from restoring a living antioxidant system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p25777939] In vitro evidence that sulfite impairs glutamatergic neurotransmission and inhibits glutathione metabolism-related enzymes in rat cerebral cortex. 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(2015). https://pubmed.ncbi.nlm.nih.gov/25777939/ DOI: 10.1016/j.ijdevneu.2015.03.005","model_system":"Sulfite/thiosulfate incubation of rat cortical slices and cell-free GSSG","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p25777939] In vitro evidence that sulfite impairs glutamatergic neurotransmission and inhibits glutathione metabolism-related enzymes in rat cerebral cortex. 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