{"id":"1b4ce034-94c8-5726-830e-6390d851c364","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-rpe-isomerization-null","predicate":"does_not_form_in_assay","statement":"This ARPE-19 delivery experiment detected no lutein conversion to meso-zeaxanthin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ab8f00f8-b624-54e6-bf50-28dc3a1bebcc","mechanism_event_label":"Uptake alone did not reproduce the separate RPE65 overexpression result.","subject":{"id":"5dd84367-3f8e-5a3f-b952-a96174ea51fa","slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"},"object":{"id":"24cea833-2dbe-5746-be4d-06b3b7c6315c","slug":"meso-zeaxanthin","display_name":"meso-Zeaxanthin / (3R,3-prime-S)-zeaxanthin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ab8f00f8-b624-54e6-bf50-28dc3a1bebcc","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-rpe-isomerization-null-event","event_type":"biochemical_relationship","label":"Uptake alone did not reproduce the separate RPE65 overexpression result.","description":"This ARPE-19 delivery experiment detected no lutein conversion to meso-zeaxanthin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5dd84367-3f8e-5a3f-b952-a96174ea51fa","slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"24cea833-2dbe-5746-be4d-06b3b7c6315c","slug":"meso-zeaxanthin","display_name":"meso-Zeaxanthin / (3R,3-prime-S)-zeaxanthin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lutein-research/27538825.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1db014221931676349f15dc3c5138fba122417fb49fec5de481594c72b0596f3\", \"start_char\": 0, \"end_char\": 1346, \"text_sha256\": \"1db014221931676349f15dc3c5138fba122417fb49fec5de481594c72b0596f3\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Carotenoid delivery using isolated human lipoproteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Carotenoid-loaded LDL versus HDL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell delivery ranking is not identical to circulating carriage fractions or the WHAM-chicken phenotype.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lutein research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human ARPE-19 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Uptake alone did not reproduce the separate RPE65 overexpression result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lutein-p27538825] Mechanisms of selective delivery of xanthophylls to retinal pigment epithelial cells by human lipoproteins. (2016). https://pubmed.ncbi.nlm.nih.gov/27538825/ DOI: 10.1194/jlr.m070193","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Retinal pigment epithelial model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7a543307-c78c-5ba6-aec0-609f09a5d382","evidence_kind":"source_excerpt","locator":"Lines 658-669","start_line":658,"end_line":669,"excerpt":"### lutein-rpe-isomerization-null\nThis ARPE-19 delivery experiment detected no lutein conversion to meso-zeaxanthin.\nCondition category: normal\nnutrient_topic: Lutein research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Uptake alone did not reproduce the separate RPE65 overexpression result.\norganism: Human ARPE-19 cells\ntissue_or_cell_type: Retinal pigment epithelial model\nexperimental_model: Carotenoid delivery using isolated human lipoproteins\nlimitations: Cell delivery ranking is not identical to circulating carriage fractions or the WHAM-chicken phenotype.\nexposure: Carotenoid-loaded LDL versus HDL\nevidence_span: {\"source_cache\": \"artifacts/lutein-research/27538825.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1db014221931676349f15dc3c5138fba122417fb49fec5de481594c72b0596f3\", \"start_char\": 0, \"end_char\": 1346, \"text_sha256\": \"1db014221931676349f15dc3c5138fba122417fb49fec5de481594c72b0596f3\"}\n[lutein-p27538825] Mechanisms of selective delivery of xanthophylls to retinal pigment epithelial cells by human lipoproteins. 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