{"id":"1b36434f-564f-5918-b2c0-a7247e802959","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-tcell-autophagy","predicate":"restores","statement":"Adding spermidine restored autophagic flux in stimulated T cells from older human donors.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"26478181-acc8-567b-bfd9-fbd402fb145d","mechanism_event_label":"The recycling response was recoverable in cultured T cells.","subject":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"object":{"id":"e3675a77-6667-5e16-a996-734f509300b6","slug":"human-aged-cd8-autophagy","display_name":"Human older-donor CD8 T-cell autophagy","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"26478181-acc8-567b-bfd9-fbd402fb145d","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-tcell-autophagy-event","event_type":"observed_relationship","label":"The recycling response was recoverable in cultured T cells.","description":"Adding spermidine restored autophagic flux in stimulated T cells from older human donors.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e3675a77-6667-5e16-a996-734f509300b6","slug":"human-aged-cd8-autophagy","display_name":"Human older-donor CD8 T-cell autophagy","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Anti-CD3/CD28-stimulated donor cells; 10 micromolar spermidine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex-vivo treatment does not establish an effective oral dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The recycling response was recoverable in cultured T cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Autophagy in T cells from aged donors is maintained by spermidine and correlates with function and vaccine responses. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33317695/ · DOI 10.7554/eLife.57950","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"97d5a1e6-51ff-5194-a057-5ffc1e37a205","evidence_kind":"source_excerpt","locator":"Lines 238-244","start_line":238,"end_line":244,"excerpt":"## spermidine-tcell-autophagy\nThe recycling response was recoverable in cultured T cells.\nAdding spermidine restored autophagic flux in stimulated T cells from older human donors.\nModel: Anti-CD3/CD28-stimulated donor cells; 10 micromolar spermidine.\nLimitations: Ex-vivo treatment does not establish an effective oral dose.\nEvidence access: Primary full text\nAutophagy in T cells from aged donors is maintained by spermidine and correlates with function and vaccine responses. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33317695/ · DOI 10.7554/eLife.57950","model_system":"Anti-CD3/CD28-stimulated donor cells; 10 micromolar spermidine.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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