{"id":"1b1e5554-653a-5a52-8461-d37f71a4276d","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-oct2","predicate":"transports","statement":"Human OCT2 mediated pH- and concentration-dependent agmatine transport, with Km 1.84 ± 0.38 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6fda77c6-a339-5f18-9c4e-0f18255f43c8","mechanism_event_label":"Another organic-cation transporter handles agmatine more avidly in this assay.","subject":{"id":"3617021d-d2f4-5199-80d7-c881b135621a","slug":"slc22a2","display_name":"Human organic cation transporter 2 / OCT2 / SLC22A2","entity_type_key":"protein"},"object":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6fda77c6-a339-5f18-9c4e-0f18255f43c8","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-oct2-event","event_type":"observed_relationship","label":"Another organic-cation transporter handles agmatine more avidly in this assay.","description":"Human OCT2 mediated pH- and concentration-dependent agmatine transport, with Km 1.84 ± 0.38 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3617021d-d2f4-5199-80d7-c881b135621a","slug":"slc22a2","display_name":"Human organic cation transporter 2 / OCT2 / SLC22A2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Matched human transporter expression systems.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Protein expression and assay conditions affect comparison with actual kidney clearance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Another organic-cation transporter handles agmatine more avidly in this assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"OCT2 and MATE1 provide bidirectional agmatine transport. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21128598/ · DOI 10.1021/mp100180a","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"pH- and concentration-dependent agmatine transport into the expressing cell.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"pH- and concentration-dependent agmatine transport into the expressing cell.","entity":null}],"evidence":[{"id":"bd3c578f-2752-50ca-ad83-deef95a14aea","evidence_kind":"source_excerpt","locator":"Lines 28-34","start_line":28,"end_line":34,"excerpt":"## agmatine-sulfate-oct2\nAnother organic-cation transporter handles agmatine more avidly in this assay.\nHuman OCT2 mediated pH- and concentration-dependent agmatine transport, with Km 1.84 ± 0.38 mM.\nModel: Matched human transporter expression systems.\nLimitations: Protein expression and assay conditions affect comparison with actual kidney clearance.\nEvidence access: Primary abstract\nOCT2 and MATE1 provide bidirectional agmatine transport. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21128598/ · DOI 10.1021/mp100180a","model_system":"Matched human transporter expression systems.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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