{"id":"1ac5bc5a-5826-5cba-bc94-5714b62061a1","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-excess-ra-hox-pattern","predicate":"alters-expression-of","statement":"Preheadfold RA exposure altered segmental Hoxb1 reporter expression in mouse hindbrain.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9684283d-c6b5-5d0f-bc86-689f35ac4a1e","mechanism_event_label":"Excess RA can shift where an embryonic patterning gene is active.","subject":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"object":{"id":"97eaeb16-8d39-5ac3-a5a8-75de142c70a1","slug":"hoxb1-gene","display_name":"Hoxb1 gene","entity_type_key":"gene"},"evidence_count":1,"mechanism_event":{"id":"9684283d-c6b5-5d0f-bc86-689f35ac4a1e","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-excess-ra-hox-pattern-event","event_type":"biochemical_relationship","label":"Excess RA can shift where an embryonic patterning gene is active.","description":"Preheadfold RA exposure altered segmental Hoxb1 reporter expression in mouse hindbrain.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ce392c9e-6483-5914-b18b-d6a2520dbbf6","slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"},"role":"nutrient-context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"97eaeb16-8d39-5ac3-a5a8-75de142c70a1","slug":"hoxb1-gene","display_name":"Hoxb1 gene","entity_type_key":"gene"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_locator","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"RA-exposed preheadfold transgenic mouse embryos; lacZ and anatomy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Experimental excess RA exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Timing-specific teratogenic experiment, not ordinary dietary intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Excess RA can shift where an embryonic patterning gene is active.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-marshall-1992] Retinoic acid alters hindbrain Hox code and induces transformation of rhombomeres 2/3 into a 4/5 identity (1992). https://www.nature.com/articles/360737a0 DOI: 10.1038/360737a0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Preheadfold embryos/hindbrain","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1df77977-1e01-5aeb-b7e6-84d50e7738d8","evidence_kind":"source_excerpt","locator":"Lines 1422-1433","start_line":1422,"end_line":1433,"excerpt":"### va-sig-excess-ra-hox-pattern\nPreheadfold RA exposure altered segmental Hoxb1 reporter expression in mouse hindbrain.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Excess RA can shift where an embryonic patterning gene is active.\norganism: Mus musculus\ntissue_or_cell_type: Preheadfold embryos/hindbrain\nexperimental_model: RA-exposed preheadfold transgenic mouse embryos; lacZ and anatomy.\nlimitations: Timing-specific teratogenic experiment, not ordinary dietary intake.\nevidence_locator: Abstract\nexposure: Experimental excess RA exposure\n[va-marshall-1992] Retinoic acid alters hindbrain Hox code and induces transformation of rhombomeres 2/3 into a 4/5 identity (1992). https://www.nature.com/articles/360737a0 DOI: 10.1038/360737a0","model_system":"RA-exposed preheadfold transgenic mouse embryos; lacZ and anatomy.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [va-marshall-1992] Retinoic acid alters hindbrain Hox code and induces transformation of rhombomeres 2/3 into a 4/5 identity (1992). https://www.nature.com/articles/360737a0 DOI: 10.1038/360737a0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}