{"id":"1a9ab854-6ba0-586e-a0f2-1df1b78a3bdb","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-microbial-selenium","predicate":"supports","statement":"Selenium supplementation was required for the increased bacterial growth yield seen with the tested Stickland amino-acid pairs; proline-related acceptors induced selenoenzyme expression.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"70c3217a-7e71-5e06-b5b0-6e486aab36bb","mechanism_event_label":"Selenium also supports some microbial amino-acid fermentation pathways.","subject":{"id":"dc89d7e2-8f89-577b-838f-42bf5d8ec7c8","slug":"selenium","display_name":"Selenium","entity_type_key":"nutrient_element"},"object":{"id":"326c90bb-dee1-5116-8b98-24dfd153a8d4","slug":"c-difficile-stickland-growth","display_name":"C. difficile growth during selenium-dependent Stickland fermentation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"70c3217a-7e71-5e06-b5b0-6e486aab36bb","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-microbial-selenium-event","event_type":"observed_relationship","label":"Selenium also supports some microbial amino-acid fermentation pathways.","description":"Selenium supplementation was required for the increased bacterial growth yield seen with the tested Stickland amino-acid pairs; 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selenite supplementation and radiolabeled selenium protein analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This bacterial growth mechanism does not establish that dietary selenium causes or prevents infection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Selenium also supports some microbial amino-acid fermentation pathways.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Analysis of proline reduction in the nosocomial pathogen Clostridium difficile. · 2006 · https://pubmed.ncbi.nlm.nih.gov/17041035/ · DOI 10.1128/JB.01370-06","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cdfd32c0-3415-5aa5-b7d1-e3d76e830205","evidence_kind":"source_excerpt","locator":"Lines 414-420","start_line":414,"end_line":420,"excerpt":"## l-proline-microbial-selenium\nSelenium also supports some microbial amino-acid fermentation pathways.\nSelenium supplementation was required for the increased bacterial growth yield seen with the tested Stickland amino-acid pairs; proline-related acceptors induced selenoenzyme expression.\nModel: Anaerobic C. difficile culture in limiting medium; selenite supplementation and radiolabeled selenium protein analysis.\nLimitations: This bacterial growth mechanism does not establish that dietary selenium causes or prevents infection.\nEvidence access: Primary abstract\nAnalysis of proline reduction in the nosocomial pathogen Clostridium difficile. · 2006 · https://pubmed.ncbi.nlm.nih.gov/17041035/ · DOI 10.1128/JB.01370-06","model_system":"Anaerobic C. difficile culture in limiting medium; selenite supplementation and radiolabeled selenium protein analysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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