{"id":"1a894283-d55e-521d-8cbe-8c1360ba93f4","stable_key":"1bbf9d44-8d2b-5b35-8f82-f6876558b0b3:cyclophilin-alone-does-not-inhibit-calcineurin","predicate":"does_not_inhibit","statement":"Cyclophilin without cyclosporin A bound did not bind or inhibit calcineurin, and neither did FKBP alone, FKBP with rapamycin, or FKBP with the inactive analogue 506BD.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"53ce8bcd-398a-56eb-b3f3-78f55a96a8a0","mechanism_event_label":"Cyclophilin without cyclosporin A bound did not bind or inhibit calcineurin, and neither did FKBP alone, FKBP with rapamycin, or FKBP with the inactive analogue 506BD.","subject":{"id":"ed602471-56bf-5b56-9137-4fe1ebffa464","slug":"cyclophilin-a","display_name":"Human cyclophilin A / PPIA","entity_type_key":"protein"},"object":{"id":"27eef74a-1d9b-57b3-a245-82d452d6cb54","slug":"calcineurin","display_name":"Calcineurin","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"53ce8bcd-398a-56eb-b3f3-78f55a96a8a0","stable_key":"1bbf9d44-8d2b-5b35-8f82-f6876558b0b3:cyclophilin-alone-does-not-inhibit-calcineurin-event","event_type":"biochemical_relationship","label":"Cyclophilin without cyclosporin A bound did not bind or inhibit calcineurin, and neither did FKBP alone, FKBP with rapamycin, or FKBP with the inactive analogue 506BD.","description":"Calcineurin phosphatase activity","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ed602471-56bf-5b56-9137-4fe1ebffa464","slug":"cyclophilin-a","display_name":"Human cyclophilin A / PPIA","entity_type_key":"protein"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"27eef74a-1d9b-57b3-a245-82d452d6cb54","slug":"calcineurin","display_name":"Calcineurin","entity_type_key":"protein_complex"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified mammalian proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Free cyclophilin; free FKBP; FKBP-rapamycin; FKBP-506BD","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is the control set that makes the complex the active species. 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No reference carries a recorded retraction, erratum or expression of concern. Each of the seven is a separate laboratory and each carries its own lineage key, so none of them can be counted twice as independent support. Study-specific concentrations, kinetic constants and limitations retained. Not publisher full text.","file_path":"","sha256":"499d00555a629cba2271c3888d7e93627949891d565c4c78c843610de2913080","revision_id":"263ee68a-4184-51d0-a977-197d7ddb7a4d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}