{"id":"1a817d6f-67d3-5f5b-b3e9-08ba827fdc35","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mouse-folate-depletion-mthfd1-enrichment","predicate":"enriches-in","statement":"Dietary folate depletion increased nuclear Mthfd1 more than twofold in mouse liver at the expense of cytosolic levels.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"9349c00a-feee-5399-80c3-0c72398f3b52","mechanism_event_label":"The enzyme redistributed toward the nucleus as folate supply fell.","subject":{"id":"cfdda38d-c2ab-533d-8d15-eb4d0239cc2f","slug":"mouse-mthfd1","display_name":"Mouse Mthfd1 protein","entity_type_key":"protein"},"object":{"id":"92a7a88e-c4dc-5d6d-babc-696ef95fff4b","slug":"nucleus","display_name":"Nucleus","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"9349c00a-feee-5399-80c3-0c72398f3b52","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mouse-folate-depletion-mthfd1-enrichment-event","event_type":"observed_intervention","label":"The enzyme redistributed toward the nucleus as folate supply fell.","description":"Dietary folate depletion increased nuclear Mthfd1 more than twofold in mouse liver at the expense of cytosolic levels.","status":"provisional","compartment":{"slug":"nucleus","display_name":"Nucleus"},"participants":[{"entity":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"role":"folate-pool representative","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cfdda38d-c2ab-533d-8d15-eb4d0239cc2f","slug":"mouse-mthfd1","display_name":"Mouse Mthfd1 protein","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"92a7a88e-c4dc-5d6d-babc-696ef95fff4b","slug":"nucleus","display_name":"Nucleus","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Dietary depletion and liver fractionation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Nuclear protection is conditional; it does not prove indefinite resistance to deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The enzyme redistributed toward the nucleus as folate supply fell.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[field-2014] Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency (2014). https://pubmed.ncbi.nlm.nih.gov/25213861/ DOI: 10.1074/jbc.m114.599589","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"29cc6b9a-d8ad-5071-84f8-f988524f2b3c","evidence_kind":"source_excerpt","locator":"Lines 961-971","start_line":961,"end_line":971,"excerpt":"### mouse-folate-depletion-mthfd1-enrichment\nDietary folate depletion increased nuclear Mthfd1 more than twofold in mouse liver at the expense of cytosolic levels.\nCondition category: nutrient_deficiency\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The enzyme redistributed toward the nucleus as folate supply fell.\norganism: Mus musculus\ntissue_or_cell_type: Liver\nexperimental_model: Dietary depletion and liver fractionation\nlimitations: Nuclear protection is conditional; it does not prove indefinite resistance to deficiency.\nexposure: Assay conditions described in the linked primary study.\n[field-2014] Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency (2014). https://pubmed.ncbi.nlm.nih.gov/25213861/ DOI: 10.1074/jbc.m114.599589","model_system":"Dietary depletion and liver fractionation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [field-2014] Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency (2014). https://pubmed.ncbi.nlm.nih.gov/25213861/ DOI: 10.1074/jbc.m114.599589","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}