{"id":"1a784deb-19e9-573e-8acf-3246b0e0f016","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-azin2-regulation","predicate":"inhibits","statement":"Human AZIN2 bound antizymes and counteracted AZ1-dependent suppression and degradation of ODC.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ff76ad6e-d706-59fa-952f-11c1da5a46fc","mechanism_event_label":"A regulatory protein can change polyamine supply without synthesizing agmatine itself.","subject":{"id":"60429969-802f-5ede-9184-a77538b844d1","slug":"azin2","display_name":"Human antizyme inhibitor 2 / AZIN2","entity_type_key":"protein"},"object":{"id":"4ee797d4-a252-5655-9662-62f1c7bc1f70","slug":"oaz1","display_name":"Human ornithine decarboxylase antizyme 1 / OAZ1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ff76ad6e-d706-59fa-952f-11c1da5a46fc","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-azin2-regulation-event","event_type":"observed_relationship","label":"A regulatory protein can change polyamine supply without synthesizing agmatine itself.","description":"Human AZIN2 bound antizymes and counteracted AZ1-dependent suppression and degradation of ODC.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"60429969-802f-5ede-9184-a77538b844d1","slug":"azin2","display_name":"Human antizyme inhibitor 2 / AZIN2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4ee797d4-a252-5655-9662-62f1c7bc1f70","slug":"oaz1","display_name":"Human ornithine decarboxylase antizyme 1 / OAZ1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f8b362af-6bb4-5955-92a8-22ade1d2a716","slug":"odc1","display_name":"Human ornithine decarboxylase / ODC1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Yeast two-hybrid, binding, ODC activity and degradation assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding and functional inhibition were studied; no oral supplement response is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A regulatory protein can change polyamine supply without synthesizing agmatine itself.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Human ornithine decarboxylase paralogue (ODCp) is an antizyme inhibitor but not an arginine decarboxylase. · 2008 · https://pubmed.ncbi.nlm.nih.gov/17900240/ · DOI 10.1042/BJ20071004","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a8a15ff5-a384-57fd-b210-a0d8002d055e","evidence_kind":"source_excerpt","locator":"Lines 180-186","start_line":180,"end_line":186,"excerpt":"## agmatine-sulfate-azin2-regulation\nA regulatory protein can change polyamine supply without synthesizing agmatine itself.\nHuman AZIN2 bound antizymes and counteracted AZ1-dependent suppression and degradation of ODC.\nModel: Yeast two-hybrid, binding, ODC activity and degradation assays.\nLimitations: Binding and functional inhibition were studied; no oral supplement response is inferred.\nEvidence access: Primary abstract\nHuman ornithine decarboxylase paralogue (ODCp) is an antizyme inhibitor but not an arginine decarboxylase. · 2008 · https://pubmed.ncbi.nlm.nih.gov/17900240/ · DOI 10.1042/BJ20071004","model_system":"Yeast two-hybrid, binding, ODC activity and degradation assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b55d70e22d07b80964f825770d92fdd1745221420ed8af8d94ca5182b9994509","revision_id":"fc7be029-20e0-5943-b74e-3b06ba694571","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}