{"id":"1a5b513f-764f-52a4-98c5-7a3e9e6a89af","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cpt2-heat","predicate":"deficiency_increases","statement":"Myocytes derived from a CPT2-deficient patient accumulated palmitoylcarnitine; incubation at 38 degrees C accentuated accumulation relative to control myocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"dc845628-48ae-5dab-babb-cbfe9097d7a3","mechanism_event_label":"A downstream block can leave more loaded carnitine behind.","subject":{"id":"ba546852-649c-5632-a58d-702bc82bb6f5","slug":"human-cpt2","display_name":"Human carnitine palmitoyltransferase 2 / CPT2","entity_type_key":"protein"},"object":{"id":"aea1b85b-584f-5fa9-8432-7461f80a29ce","slug":"human-cpt2-myocyte-c16","display_name":"Human CPT2-deficient myocyte palmitoylcarnitine accumulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dc845628-48ae-5dab-babb-cbfe9097d7a3","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cpt2-heat-event","event_type":"observed_relationship","label":"A downstream block can leave more loaded carnitine behind.","description":"Myocytes derived from a CPT2-deficient patient accumulated palmitoylcarnitine; incubation at 38 degrees C accentuated accumulation relative to control myocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ba546852-649c-5632-a58d-702bc82bb6f5","slug":"human-cpt2","display_name":"Human carnitine palmitoyltransferase 2 / CPT2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"aea1b85b-584f-5fa9-8432-7461f80a29ce","slug":"human-cpt2-myocyte-c16","display_name":"Human CPT2-deficient myocyte palmitoylcarnitine accumulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f1c881ce-8094-5602-bb97-074377340185","slug":"palmitoylcarnitine","display_name":"Palmitoyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human patient-derived iPSC myocytes; heat challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A cellular disease model, not a universal plasma threshold or a supplement trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A downstream block can leave more loaded carnitine behind.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional analysis of iPSC-derived myocytes from a patient with carnitine palmitoyltransferase II deficiency. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24780397/ · DOI 10.1016/j.bbrc.2014.04.084","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"47b18def-94d7-532c-9667-1527611bf508","evidence_kind":"source_excerpt","locator":"Lines 130-136","start_line":130,"end_line":136,"excerpt":"## l-carnitine-cpt2-heat\nA downstream block can leave more loaded carnitine behind.\nMyocytes derived from a CPT2-deficient patient accumulated palmitoylcarnitine; incubation at 38 degrees C accentuated accumulation relative to control myocytes.\nModel: Human patient-derived iPSC myocytes; heat challenge.\nLimitations: A cellular disease model, not a universal plasma threshold or a supplement trial.\nEvidence access: Primary abstract\nFunctional analysis of iPSC-derived myocytes from a patient with carnitine palmitoyltransferase II deficiency. · 2014 · https://pubmed.ncbi.nlm.nih.gov/24780397/ · DOI 10.1016/j.bbrc.2014.04.084","model_system":"Human patient-derived iPSC myocytes; heat challenge.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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