{"id":"1a49db20-19da-56a6-acc3-3c90453e405f","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-sirtuin-expression","predicate":"increases_expression","statement":"In the endothelial high-glucose experiment, ergothioneine increased SIRT1/SIRT6 expression and reduced senescence-associated responses.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"4c31ebc8-2126-52d5-9308-1aa293712069","mechanism_event_label":"Stress protection involved cell-regulatory enzymes.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"263ba437-95b0-5dd3-bf21-00b6c53c33b1","slug":"endothelial-assay-sirt6","display_name":"SIRT6 in endothelial high-glucose study, species unresolved","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4c31ebc8-2126-52d5-9308-1aa293712069","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-sirtuin-expression-event","event_type":"observed_relationship","label":"Stress protection involved cell-regulatory enzymes.","description":"In the endothelial high-glucose experiment, ergothioneine increased SIRT1/SIRT6 expression and reduced senescence-associated responses.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"263ba437-95b0-5dd3-bf21-00b6c53c33b1","slug":"endothelial-assay-sirt6","display_name":"SIRT6 in endothelial high-glucose study, species unresolved","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7dc0b10c-0146-532c-bc56-b693f38a4013","slug":"endothelial-assay-sirt1","display_name":"SIRT1 in endothelial high-glucose study, species unresolved","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"0.01-1 mM pretreatment; highest protection at 0.5 mM, then 25 mM glucose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract does not identify the endothelial species; protein nodes remain preparation-specific.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Stress protection involved cell-regulatory enzymes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ergothioneine oxidation in the protection against high-glucose induced endothelial senescence: Involvement of SIRT1 and SIRT6. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27101740/ · DOI 10.1016/j.freeradbiomed.2016.04.013","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f075ce7d-a366-54f4-ae8b-eee83b2d7aaf","evidence_kind":"source_excerpt","locator":"Lines 352-358","start_line":352,"end_line":358,"excerpt":"## ergothioneine-sirtuin-expression\nStress protection involved cell-regulatory enzymes.\nIn the endothelial high-glucose experiment, ergothioneine increased SIRT1/SIRT6 expression and reduced senescence-associated responses.\nModel: 0.01-1 mM pretreatment; highest protection at 0.5 mM, then 25 mM glucose.\nLimitations: Abstract does not identify the endothelial species; protein nodes remain preparation-specific.\nEvidence access: Primary abstract\nErgothioneine oxidation in the protection against high-glucose induced endothelial senescence: Involvement of SIRT1 and SIRT6. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27101740/ · DOI 10.1016/j.freeradbiomed.2016.04.013","model_system":"0.01-1 mM pretreatment; highest protection at 0.5 mM, then 25 mM glucose.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}