{"id":"1a37edef-1339-5c48-94c3-606b18e87b3d","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars1-charging","predicate":"charges","statement":"Human cytosolic TARS aminoacylation assays measured threonine loading onto tRNA and competitive inhibition by borrelidin-class compounds.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a4b8404f-95a4-5b7d-905c-8cb3777feee3","mechanism_event_label":"The amino acid must be attached to its matching tRNA before translation.","subject":{"id":"42b7656b-93a3-5348-a7cf-775072512653","slug":"tars1","display_name":"Human cytosolic threonyl-tRNA synthetase / TARS1","entity_type_key":"protein"},"object":{"id":"f22f14bf-fb51-54e6-948d-310dc53e8a51","slug":"human-cytosolic-threonyl-trna","display_name":"Human cytosolic threonyl-tRNA Thr","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"a4b8404f-95a4-5b7d-905c-8cb3777feee3","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars1-charging-event","event_type":"observed_relationship","label":"The amino acid must be attached to its matching tRNA before translation.","description":"Human cytosolic TARS aminoacylation assays measured threonine loading onto tRNA and competitive inhibition by borrelidin-class compounds.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"42b7656b-93a3-5348-a7cf-775072512653","slug":"tars1","display_name":"Human cytosolic threonyl-tRNA synthetase / TARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f22f14bf-fb51-54e6-948d-310dc53e8a51","slug":"human-cytosolic-threonyl-trna","display_name":"Human cytosolic threonyl-tRNA Thr","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human enzyme biochemistry alongside inhibitor structures and cellular studies.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The charging reaction and extracellular signaling by the enzyme are separate functions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The amino acid must be attached to its matching tRNA before translation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Aminoacyl-tRNA synthetase dependent angiogenesis revealed by a bioengineered macrolide inhibitor. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26271225/ · DOI 10.1038/srep13160","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c6c5f416-cda4-5904-be8a-3f45aa5f3986","evidence_kind":"source_excerpt","locator":"Lines 58-64","start_line":58,"end_line":64,"excerpt":"## l-threonine-tars1-charging\nThe amino acid must be attached to its matching tRNA before translation.\nHuman cytosolic TARS aminoacylation assays measured threonine loading onto tRNA and competitive inhibition by borrelidin-class compounds.\nModel: Human enzyme biochemistry alongside inhibitor structures and cellular studies.\nLimitations: The charging reaction and extracellular signaling by the enzyme are separate functions.\nEvidence access: Primary abstract\nAminoacyl-tRNA synthetase dependent angiogenesis revealed by a bioengineered macrolide inhibitor. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26271225/ · DOI 10.1038/srep13160","model_system":"Human enzyme biochemistry alongside inhibitor structures and cellular studies.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3365aed939d9f567bd098449c7d67c7c00fb6c9ba163dcf34dc7cf6081f324fb","revision_id":"10a7b648-8dd7-5d4e-b618-c560a6e2d3c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}