{"id":"1a36267b-e0a5-5db2-8430-b02ff550462e","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-fat-ckb-loss","predicate":"loss_reduced","statement":"Adipocyte Ckb deletion reduced thermogenic capacity and predisposed mice to obesity and disturbed glucose handling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"53c7ef90-fca2-5673-9ad8-f62246afac84","mechanism_event_label":"Removing this enzyme weakened the heat-producing pathway in mice.","subject":{"id":"e86c3ea5-b8e5-5e65-829e-41185cc4ca36","slug":"mouse-ckb","display_name":"Mouse creatine kinase B / Ckb","entity_type_key":"protein"},"object":{"id":"99246e86-63e5-544b-bcba-43869401ebef","slug":"adipocyte-thermogenesis","display_name":"Heat production and energy expenditure by thermogenic adipocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"53c7ef90-fca2-5673-9ad8-f62246afac84","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-fat-ckb-loss-event","event_type":"biochemical_relationship","label":"Removing this enzyme weakened the heat-producing pathway in mice.","description":"Adipocyte Ckb deletion reduced thermogenic capacity and predisposed mice to obesity and disturbed glucose handling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e86c3ea5-b8e5-5e65-829e-41185cc4ca36","slug":"mouse-ckb","display_name":"Mouse creatine kinase B / Ckb","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"99246e86-63e5-544b-bcba-43869401ebef","slug":"adipocyte-thermogenesis","display_name":"Heat production and energy expenditure by thermogenic adipocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/creatine-research/33597756.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"90b9ec2286a126569876b65a77450dec9f600bf04cb6f3e8c4aeb43edcc34c4b\", \"start_char\": 0, \"end_char\": 942, \"text_sha256\": \"90b9ec2286a126569876b65a77450dec9f600bf04cb6f3e8c4aeb43edcc34c4b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adipocyte biochemical experiments and conditional gene deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Thermogenic stimulation and adipocyte Ckb loss","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human cell induction and mouse causal phenotypes are separately scoped; no human weight-loss intervention was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Creatine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Removing this enzyme weakened the heat-producing pathway in mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[creatine-p33597756] Creatine kinase B controls futile creatine cycling in thermogenic fat. 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