{"id":"1a329049-f9ac-5a5b-a804-46a1227d274b","stable_key":"research:selenoo-ampylates-sdha","predicate":"ampylates","statement":"SELENOO transfers AMP from ATP onto SDHA in the reported AMPylation experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"neutral","is_public":true,"mechanism_event_id":"4bfa9cb5-2b98-5f8b-81dd-824cbfe99d38","mechanism_event_label":"SELENOO can attach an AMP group to a respiratory enzyme.","subject":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"object":{"id":"dbebfa19-a142-5d23-b8dd-e209c2dd78d5","slug":"sdha","display_name":"SDHA","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4bfa9cb5-2b98-5f8b-81dd-824cbfe99d38","stable_key":"research:selenoo-ampylates-sdha","event_type":"ampylation","label":"SELENOO can attach an AMP group to a respiratory enzyme.","description":"SELENOO transfers AMP from ATP onto SDHA in the reported AMPylation experiments.","status":"active","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dbebfa19-a142-5d23-b8dd-e209c2dd78d5","slug":"sdha","display_name":"SDHA","entity_type_key":"protein"},"role":"substrate","stoichiometry":null,"state_label":"becomes AMPylated","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"amp-donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"melanoma-related assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical substrate experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Keep this reaction separate from NAD hydrolysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mammalian","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9dd234de-e47d-518e-934a-58756fd5e219","evidence_kind":"curated_literature_summary","locator":"lines 462-472","start_line":462,"end_line":472,"excerpt":"## selenoo-ampylates-sdha\n\nSELENOO can attach an AMP group to a respiratory enzyme.\n\nSELENOO transfers AMP from ATP onto SDHA in the reported AMPylation experiments.\n\nOrganism: mammalian\nCell type: melanoma-related assays\nExperimental model: Biochemical substrate experiments\nLimitations: Keep this reaction separate from NAD hydrolysis.\nPrimary reference: [Selenoprotein O Promotes Melanoma Metastasis and Regulates Mitochondrial Complex II Activity](https://pubmed.ncbi.nlm.nih.gov/39700395/)","model_system":"Biochemical substrate experiments","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"protein-modification","plain_language":"SELENOO can attach an AMP group to a respiratory enzyme.","evidence_scope":"direct_experimental","papers":[{"key":"melo-2025-selenoo-sdha","title":"Selenoprotein O Promotes Melanoma Metastasis and Regulates Mitochondrial Complex II Activity","url":"https://pubmed.ncbi.nlm.nih.gov/39700395/","doi":"10.1158/0008-5472.CAN-23-2194","year":2025,"model":"Biochemical assays and mouse melanoma models","summary":"Identifies SDHA AMPylation and altered complex-II activity after SELENOO loss in melanoma experiments."}]}}