{"id":"1a0586f3-76be-59c7-9ced-6978f470084a","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-akt","predicate":"reduces","statement":"EGCG reduced AKT Ser473 phosphorylation alongside proliferation in the tested cancer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7fb4845f-7fd2-542a-944b-b340aa461993","mechanism_event_label":"Downstream growth signaling changed in the cell experiments.","subject":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"object":{"id":"125b83e5-e48e-5ebc-b856-ddb4c2e6d1aa","slug":"akt-ser473-phosphorylation","display_name":"AKT Ser473 phosphorylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7fb4845f-7fd2-542a-944b-b340aa461993","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-akt-event","event_type":"observed_relationship","label":"Downstream growth signaling changed in the cell experiments.","description":"EGCG reduced AKT Ser473 phosphorylation alongside proliferation in the tested cancer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"22e1b8eb-f35f-5afa-be20-b0534df9f6be","slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"125b83e5-e48e-5ebc-b856-ddb4c2e6d1aa","slug":"akt-ser473-phosphorylation","display_name":"AKT Ser473 phosphorylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"779b2149-a16f-58b8-815b-7f1f2620da1f","slug":"mtor","display_name":"Human mechanistic target of rapamycin kinase / MTOR","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"359979da-c958-50bf-b2f7-9f15e177fa5d","slug":"pi3k-family","display_name":"Phosphoinositide 3-kinase family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"MDA-MB-231 and A549 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A pathway readout does not establish a unique causal target or human anticancer efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Downstream growth signaling changed in the cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Epigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21300025/ · DOI 10.1016/j.bbrc.2011.02.010","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6a659500-93d3-50ee-9671-e79ceab689ae","evidence_kind":"source_excerpt","locator":"Lines 228-234","start_line":228,"end_line":234,"excerpt":"## egcg-akt\nDownstream growth signaling changed in the cell experiments.\nEGCG reduced AKT Ser473 phosphorylation alongside proliferation in the tested cancer cells.\nModel: MDA-MB-231 and A549 cells.\nLimitations: A pathway readout does not establish a unique causal target or human anticancer efficacy.\nEvidence access: primary abstract.\nEpigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21300025/ · DOI 10.1016/j.bbrc.2011.02.010","model_system":"MDA-MB-231 and A549 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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