{"id":"19ea5a92-20ad-5a22-9d2d-46728a997585","stable_key":"920dfea8-62e3-5bd4-8b18-d19f86eb8295:egt-training-mito-enrichment","predicate":"increases","statement":"Four weeks of endurance training enriched ergothioneine in mouse skeletal-muscle mitochondrial isolates.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"9f0292ed-db10-5549-9666-70b154688090","mechanism_event_label":"Exercise changed the mitochondrial pool, not just intake.","subject":{"id":"076e3785-b49c-5de5-93d7-9979b18a3802","slug":"mouse-voluntary-wheel-training","display_name":"Voluntary wheel endurance training in mice","entity_type_key":"cellular_process"},"object":{"id":"5212b031-332e-5aad-a1c9-ab5c771d1f9a","slug":"mouse-skeletal-mitochondrial-ergothioneine","display_name":"Ergothioneine concentration in mouse skeletal-muscle mitochondrial isolates","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9f0292ed-db10-5549-9666-70b154688090","stable_key":"920dfea8-62e3-5bd4-8b18-d19f86eb8295:egt-training-mito-enrichment-event","event_type":"biochemical_relationship","label":"Exercise changed the mitochondrial pool, not just intake.","description":"Four weeks of endurance training enriched ergothioneine in mouse skeletal-muscle mitochondrial isolates.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"076e3785-b49c-5de5-93d7-9979b18a3802","slug":"mouse-voluntary-wheel-training","display_name":"Voluntary wheel endurance training in mice","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5212b031-332e-5aad-a1c9-ab5c771d1f9a","slug":"mouse-skeletal-mitochondrial-ergothioneine","display_name":"Ergothioneine concentration in mouse skeletal-muscle mitochondrial isolates","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"measured metabolite","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Publisher abstract/introduction plus the authors' article and supplementary legends reproduced in an indexed document. This is source-derived extraction, not raw-data verification. Main-text OCR corrupts some micro-unit symbols; ambiguous doses and binding constants are deliberately not transcribed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"four weeks","comparator":"sedentary MITO-Tag mice","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"mouse-voluntary-wheel-training","display_name":"Voluntary wheel endurance training in mice","entity_type_key":"cellular_process"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"four-week voluntary wheel training\", \"comparator\": \"sedentary MITO-Tag mice\", \"endpoint\": \"muscle mitochondrial ergothioneine concentration\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": [{\"entity_slug\": \"mouse-voluntary-wheel-training\", \"state\": \"four weeks\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"MITO-Tag mice; gastrocnemius mitochondrial immunoprecipitation and targeted metabolomics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is tissue-specific metabolite enrichment, not proof of a human deficiency threshold or the transport mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine mitochondrial supplement; shared molecular requirements are not demonstrated dietary interactions.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Exercise changed the mitochondrial pool, not just intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sprenger et al. Ergothioneine controls mitochondrial function and exercise performance via direct activation of MPST. DOI 10.1016/j.cmet.2025.01.024; PMID 39965563; https://pubmed.ncbi.nlm.nih.gov/39965563/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Figure 1D-F","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e2092b88-bfe4-5ad1-929b-13af4abb4df1","evidence_kind":"source_excerpt","locator":"Lines 89-89","start_line":89,"end_line":89,"excerpt":"Four weeks of endurance training enriched ergothioneine in mouse skeletal-muscle mitochondrial isolates.","model_system":"MITO-Tag mice; gastrocnemius mitochondrial immunoprecipitation and targeted metabolomics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Evidence points to this original curation statement. Primary publication and experimental locator are separately preserved; this is not a verbatim primary-paper quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"28c5c2dc-13c0-5eb3-863c-1c4821737a7d","stable_key":"import-920dfea8-62e3-5bd4-8b18-d19f86eb8295","title":"Ergothioneine: mitochondrial transport, MPST and sulfur-handling dependencies (2026-10-02)","document_type":"imported_text","citation_label":"Original AI-assisted curation of five primary studies with publication identifiers, experimental locators and access limitations. Additive chapter supplement, not publisher full text.","file_path":"","sha256":"99efc5740185396bb599a673a0e37ef850c8ec5b07242070012981a569831220","revision_id":"eaadf18d-9dbf-5c67-97b5-d753d4dbff72","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}