{"id":"19d6887a-02b1-5a18-85e7-c4b37dbbb44f","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-allergy-transcript","predicate":"alters","statement":"SS-FA pretreatment altered allergy-associated transcripts in stimulated KU812 cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5788662a-7b71-5dff-babb-0ccbbaf32160","mechanism_event_label":"The cell response included changes in gene expression.","subject":{"id":"4402078e-d703-56fb-b9e4-37265c02cf8e","slug":"sludge-fulvic-2011","display_name":"Solubilized-sludge fulvic fraction, 2011 study","entity_type_key":"chemical_species"},"object":{"id":"21b2eed7-b42b-586d-a387-b6ac1c9c4bab","slug":"human-ku812-ssfa-transcripts","display_name":"KU812 allergy-related gene-expression profile after SS-FA","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5788662a-7b71-5dff-babb-0ccbbaf32160","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-allergy-transcript-event","event_type":"observed_relationship","label":"The cell response included changes in gene expression.","description":"SS-FA pretreatment altered allergy-associated transcripts in stimulated KU812 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4402078e-d703-56fb-b9e4-37265c02cf8e","slug":"sludge-fulvic-2011","display_name":"Solubilized-sludge fulvic fraction, 2011 study","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"21b2eed7-b42b-586d-a387-b6ac1c9c4bab","slug":"human-ku812-ssfa-transcripts","display_name":"KU812 allergy-related gene-expression profile after SS-FA","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human KU812 microarray study following PMA/calcium-ionophore stimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression changes are not proof of direct receptor binding; cell-line results do not establish an oral dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The cell response included changes in gene expression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21331654/ · DOI 10.1007/s10616-010-9333-6","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"29244400-9c6c-53a9-88e7-a48a387e657c","evidence_kind":"source_excerpt","locator":"Lines 116-122","start_line":116,"end_line":122,"excerpt":"## fulvic-acid-allergy-transcript\nThe cell response included changes in gene expression.\nSS-FA pretreatment altered allergy-associated transcripts in stimulated KU812 cells.\nModel: Human KU812 microarray study following PMA/calcium-ionophore stimulation.\nLimitations: Expression changes are not proof of direct receptor binding; cell-line results do not establish an oral dose.\nEvidence access: Primary abstract\nMicroarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21331654/ · DOI 10.1007/s10616-010-9333-6","model_system":"Human KU812 microarray study following PMA/calcium-ionophore stimulation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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