{"id":"19c66c87-d1dd-556e-ac34-687f3d510489","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:plod3-glucosylation","predicate":"glucosylates","statement":"PLOD3 transfers glucose from UDP-glucose onto galactosyl-hydroxylysine in collagen.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"682b6a94-0c54-5057-a434-310368502aa6","mechanism_event_label":"A second sugar can be added after galactose.","subject":{"id":"2b488aa8-b4d8-5d2e-bb4c-03047e3e030f","slug":"plod3","display_name":"PLOD3","entity_type_key":"protein"},"object":{"id":"9e4e923d-b82b-5f01-a370-ec4ca4309246","slug":"collagen-galactosyl-hydroxylysine","display_name":"Collagen galactosyl-hydroxylysine residues","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"682b6a94-0c54-5057-a434-310368502aa6","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:plod3-glucosylation-event","event_type":"biochemical_relationship","label":"A second sugar can be added after galactose.","description":"PLOD3 transfers glucose from UDP-glucose onto galactosyl-hydroxylysine in collagen.","status":"provisional","compartment":{"slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum"},"participants":[{"entity":{"id":"2b488aa8-b4d8-5d2e-bb4c-03047e3e030f","slug":"plod3","display_name":"PLOD3","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9e4e923d-b82b-5f01-a370-ec4ca4309246","slug":"collagen-galactosyl-hydroxylysine","display_name":"Collagen galactosyl-hydroxylysine residues","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5e63505b-1f5b-57c8-b699-60e7c6536bcd","slug":"udp-glucose","display_name":"UDP-glucose","entity_type_key":"small_molecule"},"role":"donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f832d1d-027d-51c7-945a-c07e27f474bc","slug":"udp","display_name":"UDP","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"0d503914-ca04-5570-b13e-e2e91a6916fa","slug":"collagen-glucosylgalactosyl-hydroxylysine","display_name":"Collagen glucosylgalactosyl-hydroxylysine residues","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human PLOD3-COLGALT1 complex structure and biochemical assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A second sugar can be added after galactose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[kog-glycosylation-2025] The structural basis for the human procollagen lysine hydroxylation and dual-glycosylation (2025). https://www.nature.com/articles/s41467-025-57768-9 DOI: 10.1038/s41467-025-57768-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a32dc1f6-55be-561d-8afc-4e5cb146684a","evidence_kind":"source_excerpt","locator":"Lines 457-465","start_line":457,"end_line":465,"excerpt":"### plod3-glucosylation\nPLOD3 transfers glucose from UDP-glucose onto galactosyl-hydroxylysine in collagen.\nPlain language: A second sugar can be added after galactose.\nCondition category: normal\norganism: Human\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: Human PLOD3-COLGALT1 complex structure and biochemical assays.\nlimitations: This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.\n[kog-glycosylation-2025] The structural basis for the human procollagen lysine hydroxylation and dual-glycosylation (2025). https://www.nature.com/articles/s41467-025-57768-9 DOI: 10.1038/s41467-025-57768-9","model_system":"Human PLOD3-COLGALT1 complex structure and biochemical assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [kog-glycosylation-2025] The structural basis for the human procollagen lysine hydroxylation and dual-glycosylation (2025). https://www.nature.com/articles/s41467-025-57768-9 DOI: 10.1038/s41467-025-57768-9","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}