{"id":"199d6575-7550-5f74-8c14-7c2570a2d261","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-trna-charging","predicate":"forms","statement":"GARS1 catalyzes glycyl-tRNA formation for glycine incorporation into cytosolic and mitochondrial proteins.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fed6bc15-8f63-5b62-944b-5e305acf89ef","mechanism_event_label":"A charging enzyme attaches glycine to the RNA adapter used to build protein.","subject":{"id":"64eceac0-ea3e-5c2b-b6c2-09011e4dc8e0","slug":"gars1","display_name":"Human glycyl-tRNA synthetase / GARS1","entity_type_key":"protein"},"object":{"id":"c04f28cd-c976-5954-b17b-d056288fb06c","slug":"human-glycyl-trna","display_name":"Human glycyl-tRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"fed6bc15-8f63-5b62-944b-5e305acf89ef","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-trna-charging-event","event_type":"observed_relationship","label":"A charging enzyme attaches glycine to the RNA adapter used to build protein.","description":"GARS1 catalyzes glycyl-tRNA formation for glycine incorporation into cytosolic and mitochondrial proteins.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"64eceac0-ea3e-5c2b-b6c2-09011e4dc8e0","slug":"gars1","display_name":"Human glycyl-tRNA synthetase / GARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c04f28cd-c976-5954-b17b-d056288fb06c","slug":"human-glycyl-trna","display_name":"Human glycyl-tRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Established reaction described in a primary human GARS disease study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Background enzyme chemistry is distinguished from the patient measurements; separate cytosolic/mitochondrial isoform kinetics were not measured here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A charging enzyme attaches glycine to the RNA adapter used to build protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Compound heterozygous mutations in glycyl-tRNA synthetase (GARS) cause mitochondrial respiratory chain dysfunction. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28594869/ · DOI 10.1371/journal.pone.0178125","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8825a7fd-a64f-55db-b9f7-88c0e7352eea","evidence_kind":"source_excerpt","locator":"Lines 386-392","start_line":386,"end_line":392,"excerpt":"## glycine-trna-charging\nA charging enzyme attaches glycine to the RNA adapter used to build protein.\nGARS1 catalyzes glycyl-tRNA formation for glycine incorporation into cytosolic and mitochondrial proteins.\nModel: Established reaction described in a primary human GARS disease study.\nLimitations: Background enzyme chemistry is distinguished from the patient measurements; separate cytosolic/mitochondrial isoform kinetics were not measured here.\nEvidence access: Primary abstract\nCompound heterozygous mutations in glycyl-tRNA synthetase (GARS) cause mitochondrial respiratory chain dysfunction. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28594869/ · DOI 10.1371/journal.pone.0178125","model_system":"Established reaction described in a primary human GARS disease study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}