{"id":"196d6879-a0ad-5eba-a0e0-4566c1cea2b4","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-gnmt-folate-inhibition","predicate":"inhibits","statement":"Folate pentaglutamate produced 50% inhibition of native rat GNMT at 1.3 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ec0fdf23-d3e4-5a2b-a9ba-c9925d427add","mechanism_event_label":"A folate form restrains SAM use by GNMT.","subject":{"id":"018aae56-6ae2-59cb-8d4d-6b5a37bb3025","slug":"5-methyltetrahydrofolate-pentaglutamate","display_name":"5-Methyltetrahydrofolate pentaglutamate","entity_type_key":"small_molecule"},"object":{"id":"89d2bf2f-e811-5466-bdb0-f51e12f05845","slug":"gnmt-rat","display_name":"Glycine N-methyltransferase (Rattus norvegicus)","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ec0fdf23-d3e4-5a2b-a9ba-c9925d427add","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-gnmt-folate-inhibition-event","event_type":"biochemical_relationship","label":"A folate form restrains SAM use by GNMT.","description":"Folate pentaglutamate produced 50% inhibition of native rat GNMT at 1.3 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"regulated methyl donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"018aae56-6ae2-59cb-8d4d-6b5a37bb3025","slug":"5-methyltetrahydrofolate-pentaglutamate","display_name":"5-Methyltetrahydrofolate pentaglutamate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"89d2bf2f-e811-5466-bdb0-f51e12f05845","slug":"gnmt-rat","display_name":"Glycine N-methyltransferase (Rattus norvegicus)","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Native rat liver and bacterially expressed rat GNMT.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a human circulating threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A folate form restrains SAM use by GNMT.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[luka-2008] Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate (2008). https://pubmed.ncbi.nlm.nih.gov/18501206/ DOI: 10.1016/j.bbapap.2008.04.016","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified liver enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d4af53bc-e4e0-5af6-b74c-403cc7ee2388","evidence_kind":"source_excerpt","locator":"Lines 767-776","start_line":767,"end_line":776,"excerpt":"### folate-methyl-gnmt-folate-inhibition\nFolate pentaglutamate produced 50% inhibition of native rat GNMT at 1.3 micromolar.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A folate form restrains SAM use by GNMT.\norganism: Rattus norvegicus\ntissue_or_cell_type: Purified liver enzyme\nexperimental_model: Native rat liver and bacterially expressed rat GNMT.\nlimitations: Not a human circulating threshold.\n[luka-2008] Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate (2008). https://pubmed.ncbi.nlm.nih.gov/18501206/ DOI: 10.1016/j.bbapap.2008.04.016","model_system":"Native rat liver and bacterially expressed rat GNMT.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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