{"id":"1959e44f-fac5-5ceb-9142-2e9cda6d53b4","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-lung-nrf2","predicate":"reduces_tested_abundance_of","statement":"SAC at 20 mM for 72 hours reduced Nrf2 protein in HCC827 and NCI-H1975 lung cancer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"487f0a9c-09ee-5601-8216-8a92c8f0b1d0","mechanism_event_label":"A different model showed the opposite Nrf2 response.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"487f0a9c-09ee-5601-8216-8a92c8f0b1d0","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-lung-nrf2-event","event_type":"observed_relationship","label":"A different model showed the opposite Nrf2 response.","description":"SAC at 20 mM for 72 hours reduced Nrf2 protein in HCC827 and NCI-H1975 lung cancer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"10 or 20 mM SAC; 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species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A different model showed the opposite Nrf2 response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[33136700] S-allylcysteine induces cytotoxic effects in two human lung cancer cell lines via induction of oxidative damage, downregulation of Nrf2 and NF-κB, and apoptosis. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33136700/ · DOI 10.1097/CAD.0000000000001015","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Cell culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HCC827 and NCI-H1975 lung cancer cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e6329749-0e96-51b1-89f1-1a8cc9d95b08","evidence_kind":"source_excerpt","locator":"Lines 311-318","start_line":311,"end_line":318,"excerpt":"## s-allylcysteine-lung-nrf2\nA different model showed the opposite Nrf2 response.\nSAC at 20 mM for 72 hours reduced Nrf2 protein in HCC827 and NCI-H1975 lung cancer cells.\nModel: HCC827 and NCI-H1975 lung cancer cells\nLimitations: Very high concentration in malignant cells; not a contradiction of lower-exposure or different-tissue observations.\nEvidence access: Primary abstract\n[33136700] S-allylcysteine induces cytotoxic effects in two human lung cancer cell lines via induction of oxidative damage, downregulation of Nrf2 and NF-κB, and apoptosis. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33136700/ · DOI 10.1097/CAD.0000000000001015\nStructured context: {\"organism\": \"Homo sapiens\", \"tissue_or_cell_type\": \"HCC827 and NCI-H1975 lung cancer cells\", \"dose\": \"10 or 20 mM SAC; mechanistic endpoints 20 mM\", \"duration\": \"24, 48 or 72 hours; mechanistic endpoints 72 hours\", \"route\": \"Cell culture\", \"experimental_comparison\": \"SAC; BEAS-2B and A549 comparator cell lines also studied\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"HCC827 and NCI-H1975 lung cancer cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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