{"id":"1944c9bb-e501-56fe-a2d6-6d1c2bbcfcc9","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-rat-polyamine-competition","predicate":"competitively_inhibits","statement":"Agmatine present in culture medium competitively inhibited radiolabeled polyamine uptake, increasing apparent Km.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b0a10ddd-5f60-5513-81be-8949bb61c616","mechanism_event_label":"Immediate transport competition can coexist with slower regulation of protein expression.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"5cc4aef5-e828-5128-b06b-165b2cfd5f8c","slug":"rat-pulmonary-endothelial-polyamine-uptake","display_name":"Rat pulmonary-endothelial polyamine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b0a10ddd-5f60-5513-81be-8949bb61c616","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-rat-polyamine-competition-event","event_type":"observed_relationship","label":"Immediate transport competition can coexist with slower regulation of protein expression.","description":"Agmatine present in culture medium competitively inhibited radiolabeled polyamine uptake, increasing apparent Km.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5cc4aef5-e828-5128-b06b-165b2cfd5f8c","slug":"rat-pulmonary-endothelial-polyamine-uptake","display_name":"Rat pulmonary-endothelial polyamine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c71bf622-f14b-5314-b1a5-dfa002d2d8ff","slug":"putrescine","display_name":"Putrescine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"ded80d0b-9076-5034-9e87-cd38f7dafae5","slug":"spermine","display_name":"Spermine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat pulmonary endothelial transport kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No universal human polyamine depletion magnitude or clinical supplement interaction is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Immediate transport competition can coexist with slower regulation of protein expression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Regulation of ornithine decarboxylase activity and polyamine transport by agmatine in rat pulmonary artery endothelial cells. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11160620/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"83c666a6-ad9b-5d71-8448-0bfcda39a81c","evidence_kind":"source_excerpt","locator":"Lines 364-370","start_line":364,"end_line":370,"excerpt":"## agmatine-sulfate-rat-polyamine-competition\nImmediate transport competition can coexist with slower regulation of protein expression.\nAgmatine present in culture medium competitively inhibited radiolabeled polyamine uptake, increasing apparent Km.\nModel: Rat pulmonary endothelial transport kinetics.\nLimitations: No universal human polyamine depletion magnitude or clinical supplement interaction is established.\nEvidence access: Primary abstract\nRegulation of ornithine decarboxylase activity and polyamine transport by agmatine in rat pulmonary artery endothelial cells. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11160620/","model_system":"Rat pulmonary endothelial transport kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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