{"id":"193cc80c-c419-5c00-a21f-2dff461e7bf1","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars1-secretion","predicate":"is_secreted_after","statement":"Human endothelial cells secreted TARS after TNF-alpha or VEGF exposure.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aa942719-4d4e-5ee9-a68d-25bc235af0a5","mechanism_event_label":"A protein that loads threonine can also leave the cell under signaling conditions.","subject":{"id":"42b7656b-93a3-5348-a7cf-775072512653","slug":"tars1","display_name":"Human cytosolic threonyl-tRNA synthetase / TARS1","entity_type_key":"protein"},"object":{"id":"a0f5ef77-0995-5cba-bf11-9b6e86967c1e","slug":"human-endothelial-tnf-vegf-stimulation","display_name":"Human endothelial TNF-alpha or VEGF stimulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"aa942719-4d4e-5ee9-a68d-25bc235af0a5","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars1-secretion-event","event_type":"observed_relationship","label":"A protein that loads threonine can also leave the cell under signaling conditions.","description":"Human endothelial cells secreted TARS after TNF-alpha or VEGF exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"42b7656b-93a3-5348-a7cf-775072512653","slug":"tars1","display_name":"Human cytosolic threonyl-tRNA synthetase / TARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a0f5ef77-0995-5cba-bf11-9b6e86967c1e","slug":"human-endothelial-tnf-vegf-stimulation","display_name":"Human endothelial TNF-alpha or VEGF stimulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human vascular endothelial cell culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is secretion of the enzyme, not secretion of threonine or proof that dietary threonine triggers the process.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A protein that loads threonine can also leave the cell under signaling conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Secreted Threonyl-tRNA synthetase stimulates endothelial cell migration and angiogenesis. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23425968/ · DOI 10.1038/srep01317","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3f7c3872-d8a1-5e9e-b715-7afb824e2b80","evidence_kind":"source_excerpt","locator":"Lines 378-384","start_line":378,"end_line":384,"excerpt":"## l-threonine-tars1-secretion\nA protein that loads threonine can also leave the cell under signaling conditions.\nHuman endothelial cells secreted TARS after TNF-alpha or VEGF exposure.\nModel: Human vascular endothelial cell culture.\nLimitations: This is secretion of the enzyme, not secretion of threonine or proof that dietary threonine triggers the process.\nEvidence access: Primary abstract\nSecreted Threonyl-tRNA synthetase stimulates endothelial cell migration and angiogenesis. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23425968/ · DOI 10.1038/srep01317","model_system":"Human vascular endothelial cell culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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