{"id":"19029314-d475-5867-bf93-09e18285cb08","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-gsh-depletion","predicate":"depletes","statement":"Activated human polymorphonuclear leukocytes exposed to 100 micromolar eugenol lost about 90% of intracellular glutathione over 30 minutes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"94fe6390-a532-5131-9ca1-5ff4a061fc2a","mechanism_event_label":"Activated human polymorphonuclear leukocytes exposed to 100 micromolar eugenol lost about 90% of intracellular glutathione over 30 minutes.","subject":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"object":{"id":"07224f77-2258-5f24-bbb9-1a4b2fbf2eaf","slug":"human-pmn-glutathione-pool","display_name":"Glutathione pool in activated human polymorphonuclear leukocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"94fe6390-a532-5131-9ca1-5ff4a061fc2a","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-gsh-depletion-event","event_type":"observed_relationship","label":"Activated human polymorphonuclear leukocytes exposed to 100 micromolar eugenol lost about 90% of intracellular glutathione over 30 minutes.","description":"Activated human polymorphonuclear leukocytes exposed to 100 micromolar eugenol lost about 90% of intracellular glutathione over 30 minutes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"07224f77-2258-5f24-bbb9-1a4b2fbf2eaf","slug":"human-pmn-glutathione-pool","display_name":"Glutathione pool in activated human polymorphonuclear leukocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol 100 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"30 minutes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Phorbol-ester-stimulated human polymorphonuclear leukocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The large loss required activated cells and a high direct exposure; it is not evidence that culinary cloves cause systemic glutathione deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Phorbol-ester-stimulated human polymorphonuclear leukocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Activated human polymorphonuclear leukocytes exposed to 100 micromolar eugenol lost about 90% of intracellular glutathione over 30 minutes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic activation of eugenol by myeloperoxidase and polymorphonuclear leukocytes. (1989). https://pubmed.ncbi.nlm.nih.gov/2562421/ DOI: 10.1021/tx00009a011","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Intracellular glutathione and oxidative burst","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9d8c3c59-fe65-5d06-8355-bd425084a089","evidence_kind":"source_excerpt","locator":"Lines 99-108","start_line":99,"end_line":108,"excerpt":"## eugenol-gsh-depletion\nActivated human polymorphonuclear leukocytes exposed to 100 micromolar eugenol lost about 90% of intracellular glutathione over 30 minutes.\nModel/species: Phorbol-ester-stimulated human polymorphonuclear leukocytes\nTissue/system: Intracellular glutathione and oxidative burst\nExposure: Eugenol 100 micromolar\nRoute: In vitro\nDuration: 30 minutes\nLimits: The large loss required activated cells and a high direct exposure; it is not evidence that culinary cloves cause systemic glutathione deficiency.\nPrimary reference: Metabolic activation of eugenol by myeloperoxidase and polymorphonuclear leukocytes. (1989). https://pubmed.ncbi.nlm.nih.gov/2562421/ DOI: 10.1021/tx00009a011\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Phorbol-ester-stimulated human polymorphonuclear leukocytes","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}