{"id":"18c1b985-ae40-5a70-8087-7204bbcf768c","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-rat-depletion","predicate":"when_depleted_reduces","statement":"A phenylalanine/tyrosine-free amino-acid injection mixture reduced tyrosine in rat prefrontal cortex and nucleus accumbens.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ec92e22f-a3b0-5c5f-b423-a3cfb1cbcf33","mechanism_event_label":"Experimental precursor depletion can reduce local availability.","subject":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"object":{"id":"53ee8d42-2621-56ce-9cc9-b74ea832349f","slug":"rat-brain-tyrosine-after-aptd","display_name":"Rat prefrontal/nucleus-accumbens tyrosine after combined precursor depletion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ec92e22f-a3b0-5c5f-b423-a3cfb1cbcf33","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-rat-depletion-event","event_type":"observed_relationship","label":"Experimental precursor depletion can reduce local availability.","description":"A phenylalanine/tyrosine-free amino-acid injection mixture reduced tyrosine in rat prefrontal cortex and nucleus accumbens.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"53ee8d42-2621-56ce-9cc9-b74ea832349f","slug":"rat-brain-tyrosine-after-aptd","display_name":"Rat prefrontal/nucleus-accumbens tyrosine after combined precursor depletion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat amino-acid injection versus control mixture or saline; tissue HPLC.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Combined depletion is not isolated dietary tyrosine deficiency. Correction record: The 2018 correction (PMID 30094500) reports an error in norepinephrine standard concentrations used for HPLC calibration. The correction abstract was inspected, but full notice content was unavailable; its complete quantitative scope remains unverified. This collection uses the tyrosine depletion and dopamine-transient findings and does not reuse the original norepinephrine concentration result. https://pubmed.ncbi.nlm.nih.gov/30094500/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Experimental precursor depletion can reduce local availability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Acute phenylalanine/tyrosine depletion of phasic dopamine in the rat brain. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26944052/ · DOI 10.1007/s00213-016-4259-0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"b9cc726f-6b57-5110-9862-9b62a0933d3e","evidence_kind":"source_excerpt","locator":"Lines 380-386","start_line":380,"end_line":386,"excerpt":"## l-tyrosine-rat-depletion\nExperimental precursor depletion can reduce local availability.\nA phenylalanine/tyrosine-free amino-acid injection mixture reduced tyrosine in rat prefrontal cortex and nucleus accumbens.\nModel: Rat amino-acid injection versus control mixture or saline; tissue HPLC.\nLimitations: Combined depletion is not isolated dietary tyrosine deficiency. Correction record: The 2018 correction (PMID 30094500) reports an error in norepinephrine standard concentrations used for HPLC calibration. The correction abstract was inspected, but full notice content was unavailable; its complete quantitative scope remains unverified. This collection uses the tyrosine depletion and dopamine-transient findings and does not reuse the original norepinephrine concentration result. https://pubmed.ncbi.nlm.nih.gov/30094500/\nEvidence access: Primary abstract\nAcute phenylalanine/tyrosine depletion of phasic dopamine in the rat brain. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26944052/ · DOI 10.1007/s00213-016-4259-0","model_system":"Rat amino-acid injection versus control mixture or saline; tissue HPLC.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7777f4440a8bfb14a04ff73e73392ccc45d8ee89f7853ecbcc0027d6f563b8a0","revision_id":"e1206111-3a75-5809-b8e9-231ff809ff1e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}