{"id":"18c0d36e-f566-5982-9c62-4c309f7a309c","stable_key":"c8c7aa13-14c6-5f5c-9afc-9217d0f2f736:hfcs-khk-phosphorylation","predicate":"phosphorylates_fructose_to","statement":"Purified human liver ketohexokinase catalyzed the ATP-dependent phosphorylation of fructose.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"493be512-c272-526c-816d-ba060f9ccbd5","mechanism_event_label":"Purified human liver ketohexokinase catalyzed the ATP-dependent phosphorylation of fructose.","subject":{"id":"599b478d-692a-5baa-a176-ea5f55769e74","slug":"khk","display_name":"Human ketohexokinase / KHK","entity_type_key":"protein"},"object":{"id":"a273c7f5-e73a-5464-8947-f2d52f201d88","slug":"fructose-1-phosphate","display_name":"D-Fructose 1-phosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"493be512-c272-526c-816d-ba060f9ccbd5","stable_key":"c8c7aa13-14c6-5f5c-9afc-9217d0f2f736:hfcs-khk-phosphorylation-event","event_type":"observed_relationship","label":"Purified human liver ketohexokinase catalyzed the ATP-dependent phosphorylation of fructose.","description":"Purified human liver ketohexokinase catalyzed the ATP-dependent phosphorylation of fructose.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"599b478d-692a-5baa-a176-ea5f55769e74","slug":"khk","display_name":"Human ketohexokinase / KHK","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a273c7f5-e73a-5464-8947-f2d52f201d88","slug":"fructose-1-phosphate","display_name":"D-Fructose 1-phosphate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3c379840-5688-5b5d-84c0-5950b4fd8f17","slug":"fructose","display_name":"Fructose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"136c3764-1b93-5f79-8673-9001cab9bc3d","slug":"adp","display_name":"Adenosine diphosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Fructose substrate and ATP-dependent enzyme characterization; exact concentrations not in abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Assay duration not recovered","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract/metadata; unrecovered methods explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ketohexokinase purified from human liver","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Human component biochemistry","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Isoform unresolved; enzyme chemistry does not quantify whole-body flux after ordinary HFCS intake. Only abstract/metadata recovered for this scanned article.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"HFCS chapter: actual formulation studies, component biochemistry and interventions are explicitly distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"high-fructose-corn-syrup","display_name":"High-Fructose Corn Syrup / HFCS","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Ketohexokinase purified from human liver","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Purified human liver ketohexokinase catalyzed the ATP-dependent phosphorylation of fructose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The purification and properties of human liver ketohexokinase. A role for ketohexokinase and fructose-bisphosphate aldolase in the metabolic production of oxalate from xylitol. (1985). https://pubmed.ncbi.nlm.nih.gov/2996495/ DOI: 10.1042/bj2300053","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro enzyme/substrate incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Cell-free enzyme kinetics","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"29cd7c41-fb2c-5d81-83c1-49a9736083b0","evidence_kind":"source_excerpt","locator":"Lines 101-111","start_line":101,"end_line":111,"excerpt":"## hfcs-khk-phosphorylation\nPurified human liver ketohexokinase catalyzed the ATP-dependent phosphorylation of fructose.\nModel/species: Ketohexokinase purified from human liver\nTissue: Cell-free enzyme kinetics\nExposure: Fructose substrate and ATP-dependent enzyme characterization; exact concentrations not in abstract\nRoute: In vitro enzyme/substrate incubation\nDuration: Assay duration not recovered\nExposure scope: Human component biochemistry\nLimits: Isoform unresolved; enzyme chemistry does not quantify whole-body flux after ordinary HFCS intake. Only abstract/metadata recovered for this scanned article.\nReference: The purification and properties of human liver ketohexokinase. A role for ketohexokinase and fructose-bisphosphate aldolase in the metabolic production of oxalate from xylitol. (1985). https://pubmed.ncbi.nlm.nih.gov/2996495/ DOI: 10.1042/bj2300053\nAccess: Primary abstract/metadata; unrecovered methods explicitly retained.","model_system":"Ketohexokinase purified from human liver","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e863ddf6-7c1e-5a70-8335-7f3ac3eeee92","stable_key":"import-c8c7aa13-14c6-5f5c-9afc-9217d0f2f736","title":"High-Fructose Corn Syrup: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twenty primary studies and official FDA composition information, with one reused canonical glucose-transport claim. Study-specific citations, negative findings and limitations retained. 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