{"id":"189bd2ff-81c3-5b75-b93c-7ada4603bd50","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-mct1-capacity","predicate":"supports","statement":"After preincubation with 2 mM sodium butyrate, AA/C1 cells increased the maximal butyrate uptake rate about fivefold without a significant change in apparent Km.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c27613a7-91e5-5050-a8ca-393821b9373c","mechanism_event_label":"Transport capacity increased rather than measured substrate affinity.","subject":{"id":"41609998-4c7b-51bc-bc6e-fd2f45c3cee8","slug":"slc16a1","display_name":"Human monocarboxylate transporter 1 / SLC16A1","entity_type_key":"protein"},"object":{"id":"64252ef6-7c07-5d73-8726-048b0bc9316c","slug":"human-colonic-butyrate-uptake","display_name":"Human colonic-cell butyrate transport","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c27613a7-91e5-5050-a8ca-393821b9373c","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-mct1-capacity-event","event_type":"observed_relationship","label":"Transport capacity increased rather than measured substrate affinity.","description":"After preincubation with 2 mM sodium butyrate, AA/C1 cells increased the maximal butyrate uptake rate about fivefold without a significant change in apparent Km.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"41609998-4c7b-51bc-bc6e-fd2f45c3cee8","slug":"slc16a1","display_name":"Human monocarboxylate transporter 1 / SLC16A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"64252ef6-7c07-5d73-8726-048b0bc9316c","slug":"human-colonic-butyrate-uptake","display_name":"Human colonic-cell butyrate transport","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4baf6011-dcc4-5a69-9c77-a21de6060383","slug":"sodium-butyrate","display_name":"Sodium butyrate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human colonic-cell radiotracer uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An adaptive culture response does not establish oral bioavailability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; 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evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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